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Updated: Feb 14, 2026

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
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Preparation of Antisense Oligonucleotides to Inhibit miRNA Function

Chengjian Li, Phillip D Zamore

    Cold Spring Harbor Protocols
    |February 14, 2018
    PubMed
    Summary

    This protocol details designing antisense oligonucleotides (ASOs) to inhibit microRNA (miRNA) function in cells. Modifications like 2'-O-methyl and cholesterol conjugation improve ASO effectiveness and cellular uptake.

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