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Updated: Feb 14, 2026

Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
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A CsMYB6-CsTRY module regulates fruit trichome initiation in cucumber.

Sen Yang1, Yanling Cai1, Xingwang Liu1

  • 1Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, China.

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|February 14, 2018
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Summary

Cucumber fruit spine development is regulated by the CsMYB6 gene, which acts with CsTRY to negatively control trichome formation. This study reveals a novel molecular mechanism for fruit epidermal traits.

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Area of Science:

  • Plant molecular biology
  • Genetics and genomics
  • Agricultural science

Background:

  • Fruit epidermal features like trichomes and spines are crucial for cucumber quality.
  • The molecular mechanisms governing cucumber fruit spine development are largely unknown.

Purpose of the Study:

  • To functionally characterize the cucumber CsMYB6 gene and its role in fruit trichome development.
  • To elucidate the molecular pathway regulating fruit spine formation in cucumber.

Main Methods:

  • Spatial-temporal gene expression analysis of CsMYB6 and CsTRY.
  • Gene overexpression studies in cucumber and Arabidopsis.
  • Analysis of CsMYB6 binding to the CsTRY promoter.
  • Protein-protein interaction assays between CsMYB6 and CsTRY.

Main Results:

  • CsMYB6 is highly expressed during cucumber fruit spine initiation.
  • Both CsMYB6 and CsTRY negatively regulate trichome initiation in cucumber and Arabidopsis.
  • CsMYB6 acts upstream of CsTRY, binding to its promoter and interacting with its protein.
  • Conserved and divergent roles of CsMYB6 and its Arabidopsis homolog AtMYB106 were observed.

Conclusions:

  • The CsMYB6-CsTRY complex negatively regulates fruit trichome formation in cucumber.
  • This study uncovers a novel mechanism controlling a key fruit quality trait in cucumber.
  • Findings provide insights into the conserved and specific roles of MYB transcription factors in plant trichome development.