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Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
Published on: June 5, 2015
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Open-source recombinant monoclonal secondary nanobodies
1Institut für Biochemie, Freie Universität Berlin, Berlin, Germany helge.ewers@fu-berlin.de.
The Journal of Cell Biology
|February 16, 2018
Summary
Researchers developed novel nanobodies, which are single domain antibody fragments, for use as secondary antibodies. These recombinant nanobodies show promise for Western blotting, ELISA, and immunofluorescence assays in biomedical research.
Area of Science:
- Biotechnology
- Immunology
- Molecular Biology
Background:
- Secondary antibodies are crucial reagents in biomedical research.
- Traditional secondary antibodies are generated in animals, posing production and specificity challenges.
Purpose of the Study:
- To develop and characterize novel single domain antibody fragments, known as nanobodies, as alternatives to traditional secondary antibodies.
- To assess the utility of these nanobodies in common immunological assays.
Main Methods:
- Recombinant production of single domain antibody fragments (nanobodies) targeting antibodies from mouse and rabbit.
- Validation of nanobody performance in Western blotting, enzyme-linked immunosorbent assay (ELISA), and immunofluorescence assays.
Main Results:
- Successfully generated and characterized nanobodies against mouse and rabbit antibodies.
- Demonstrated the efficacy of these nanobodies in Western blotting, ELISA, and immunofluorescence.
Conclusions:
- Recombinant nanobodies offer a viable and easily produced alternative to conventional secondary antibodies.
- These nanobodies are effective tools for various immunological detection methods in research.
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