Related Experiment Video

Updated: Feb 14, 2026

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
10:31

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

Published on: February 3, 2022

3.5K

Prevalence of proline racemase/ hydroxyproline epimerase gene in human brucella isolates in Iran

Iman Hashemifar1, Faramarz Masjedian Jazi1, Abbas Yadegar2

  • 1. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Medical Journal of the Islamic Republic of Iran
|February 16, 2018
PubMed

Insights

Most Iranian Brucella isolates carry the prpA gene, a key virulence factor. This finding highlights the gene

Area of Science:

  • Microbiology
  • Immunology
  • Genetics

Background:

  • Human brucellosis is a zoonotic disease caused by Brucella species.
  • Brucella infections can lead to chronic illness by evading host immune defenses.

Purpose of the Study:

  • To determine the prevalence of the prpA gene in human Brucella isolates.
  • Investigate the role of prpA as a virulence determinant in Brucella.

Main Methods:

  • Standardized a Polymerase Chain Reaction (PCR) assay for prpA detection.
  • Designed and validated primers for the prpA gene using bioinformatics.
  • Analyzed 37 Brucella isolates from human specimens, including DNA sequencing.

Main Results:

  • The prpA gene was detected in 32 out of 37 (86.4%) Brucella isolates.
  • Positive isolates included 31 Brucella melitensis and 6 Brucella abortus strains.

Conclusions:

  • The high prevalence of the prpA gene in Iranian Brucella isolates suggests its significant role in pathogenicity.
  • This finding underscores the importance of prpA in the immune modulation strategies of Brucella.
Keywords:
Brucella speciesBrucellosisProline RacemaseprpA

More Related Videos

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells15:29

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Published on: August 5, 2016

8.6K
Gene Expression Analyses in Human Follicles09:09

Gene Expression Analyses in Human Follicles

Published on: February 17, 2023

1.5K

Related Concept Videos

Prevalence and Incidence01:08

Prevalence and Incidence

In statistical epidemiology and health sciences, two essential metrics—prevalence and incidence—are fundamental for understanding disease dynamics within a population. These measures enable public health officials, epidemiologists, and researchers to assess the burden of diseases, allocate resources effectively, and design impactful public health policies and interventions.
Prevalence indicates the proportion of individuals in a population who have a specific disease or health...
1.9K
Organization of Genes02:07

Organization of Genes

Overview
73.7K
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.7K
Gene Flow02:39

Gene Flow

Gene flow is the transfer of genes among populations, resulting from either the dispersal of gametes or from the migration of individuals.
38.1K
Gene Families01:57

Gene Families

Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
10.0K
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
10.7K