Related Experiment Video
Updated: Feb 14, 2026
10:24
Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface
Published on: July 4, 2018
12.3K
Bacterial microleakage at the abutment-implant interface, in vitro study
Carlos Larrucea1, Aparicio Conrado2, Denise Olivares1
1Postgrado de Rehabilitación Oral, Universidad de Talca, Talca, Chile.
Clinical Implant Dentistry and Related Research
|February 16, 2018
Summary
Applying sufficient torque to implant abutments prevents bacterial leakage. Torques of 20 and 30 N ensured no microleakage at the abutment-implant interface (AII), unlike lower torques.
Area of Science:
- Dental Implantology
- Biomaterials Science
- Microbiology
Background:
- A microspace at the abutment-implant interface (AII) in dental implants can harbor oral microbiome, leading to peri-implant inflammation.
- Preventing microbial leakage through the AII is crucial for maintaining peri-implant tissue health.
Purpose of the Study:
- To investigate the effect of applied torque on the prosthetic implant on marginal bacterial microleakage at the AII.
- To establish a threshold for secure abutment-implant connections against bacterial ingress.
Main Methods:
- Twenty-five internal conical implants were subjected to varying torques (<10, 10, 20, 30, 30 N) for abutment connection.
- Samples underwent occlusal loading and thermocycling, followed by micro-computed tomography (micro-CT) analysis and a bacterial leakage model using Porphyromonas gingivalis.
Main Results:
- Bacterial leakage was detected exclusively in implants subjected to <10 N and 10 N torque.
- Micro-CT analysis revealed poor abutment/implant adjustment in the <10 N and 10 N groups.
- Higher torques (20 N and 30 N) demonstrated no bacterial leakage.
Conclusions:
- The torque applied to the abutment-implant system significantly influences bacterial leakage at the AII.
- A minimum torque of 20 N is recommended to prevent marginal bacterial microleakage and ensure implant stability.
Related Concept Videos
02:04Protein-protein Interfaces
14.8K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.8K
02:04Protein-Protein Interfaces
4.5K
4.5K
01:30Bacterial Signaling
41.4K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
41.4K
01:33Bacterial Transformation
60.2K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
60.2K
01:16Methods for Studying Drug Absorption: In vitro
646
In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
646
01:16In-vitro Mutagenesis
16.7K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
16.7K