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Keith H Parsons1, Mosharrof H Mondal2, Charles L McCormick1,3

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A synthetic polymer effectively delivers RNA interference (RNAi) agents to pests like Spodoptera frugiperda, overcoming biological barriers. This breakthrough enables broader application of RNAi technology for targeted and safe pest control.

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Area of Science:

  • Biotechnology
  • Entomology
  • Materials Science

Background:

  • RNA interference (RNAi) offers species-specific pest control but faces biological barriers in some insects.
  • Developing effective delivery methods is crucial for widespread RNAi insecticide application.

Purpose of the Study:

  • To investigate the efficacy of a synthetic cationic polymer, pGPMA, in facilitating RNAi in the pest Spodoptera frugiperda.
  • To assess the potential of polymer-dsRNA complexes for oral delivery and gene knockdown in insect pests.

Main Methods:

  • Synthesized poly-[N-(3-guanidinopropyl)methacrylamide] (pGPMA) to mimic cell-penetrating peptides.
  • Formulated polymer-dsRNA interpolyelectrolyte complexes (IPECs).
  • Tested IPEC uptake, gene knockdown efficiency, and larval mortality in Spodoptera frugiperda.

Main Results:

  • pGPMA-dsRNA IPECs were efficiently internalized by cells, leading to high gene knockdown.
  • Oral administration of IPECs resulted in target gene knockdown and moderate larval mortality in S. frugiperda.
  • Observed sequence-specific effects and low polymer toxicity, indicating safety.

Conclusions:

  • pGPMA effectively triggers RNAi in insects resistant to conventional methods.
  • This polymer-based delivery system shows promise for oral RNAi insecticide development.
  • The technology can expand the utility of RNAi in integrated pest management strategies.