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Updated: Feb 14, 2026

Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT
Published on: September 2, 2015
Distinct photophysical behaviour and transport of cell-impermeable [Ru(bpy)2dppz]2+ in live cells using
Meenakshi N Shinde1, Soniya S Rao, Shridhar P Gejji
1Department of Chemistry, Savitribai Phule Pune University, Pune-411 007, India. aak@chem.unipune.ac.in.
Abstract:
Here, we report the delivery of a cell-impermeable [Ru(bpy)2dppz]2+ complex across a cell membrane using a cucurbit[7]uril molecular container. Encapsulation of complex 1 in the cucurbit[7]uril cavity showed an 830-fold enhancement in the luminescence intensity of the non-emissive complex in aqueous solution. This molecular light-switch effect stems from the incorporation of the dppz ligand of 1 inside the CB7 cavity and can be attributed to long range coulombic forces between Ru2+ and the carbonyl portal of CB7 via CHO interactions. This is reflected in the 1H-NMR experiments, and further corroborated by theoretical calculations.
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