Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Graded Potential01:19

Graded Potential

7.1K
Graded potentials are localized fluctuations in the cell membrane's electrical charge, commonly found in the dendrites of neurons. The magnitude of these potential changes depends on the strength of the initiating stimulus. In a membrane at its resting potential, a graded potential signifies a voltage shift either above -70 mV or below -70 mV.
Graded potentials fall into two categories: depolarizing and hyperpolarizing. Depolarizing graded potentials typically occur when sodium (Na+) or...
7.1K
Inflammation01:38

Inflammation

62.4K
Overview
62.4K
Types of Aggregate Grading01:15

Types of Aggregate Grading

1.5K
Aggregate grading is crucial in economically obtaining a concrete mix with adequate strength, reasonable workability, and minimal segregation. There are four types of aggregate gradation: well-graded, uniformly (or one-sized) graded, gap-graded, and open-graded.
Well-graded aggregates include a complete range of necessary size fractions that fit together to create a dense matrix with minimal voids, represented by a smooth, continuous gradation curve. This type of grading ensures good...
1.5K
Sieve Analysis and Grading Curves01:19

Sieve Analysis and Grading Curves

1.0K
Sieve analysis is a method used to determine the particle size distribution of aggregate materials. This process involves the following steps:
1.0K
Potential Energy00:52

Potential Energy

42.9K
The energy stored by a structure and location of matter in space is called potential energy. For instance, raising a kettlebell changes its spatial location and increases its potential energy. Similarly, a stretched rubber band contains potential energy which, under certain conditions, can be converted into other forms of energy, such as kinetic energy.
Chemical bonds that form attractive forces between atoms also contain potential energy, called chemical energy. When a chemical reaction...
42.9K
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

13.8K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
13.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Injured epithelial cell states impact kidney allograft survival after T-cell-mediated rejection.

Nature communications·2026
Same author

Transanal total mesorectal excision versus laparoscopic total mesorectal excision for mid and low rectal cancer (COLOR III): short-term outcomes of an international, multicentre, phase 3, randomised, controlled, non-inferiority trial.

The lancet. Gastroenterology & hepatology·2026
Same author

Injured epithelial cell states impact kidney allograft survival after T-cell-mediated rejection.

Nature communications·2026
Same author

Artificial intelligence classification of rectal neoplasia by endoscopic fluorescence perfusion analysis.

Scientific reports·2026
Same author

Lipocalin-2 modulates recipients alloimmune responses to the murine kidney transplants.

Frontiers in immunology·2026
Same author

Salvage Surgery After Non-Curative Endoscopic Submucosal Dissection for Early Colorectal Cancer: A Comprehensive Review.

Journal of clinical medicine·2025
Same journal

Optimized intracellular flow cytometry panel enables CD4 and CD8 T cell cytokine profiling in Syrian hamsters.

Journal of immunological methods·2026
Same journal

Isosulfan blue sentinel lymph node biopsy enables reliable lymph node harvest and multicolor flow cytometry in mice.

Journal of immunological methods·2026
Same journal

Type-specific antibody detection of herpes simplex virus types 1&2 (HSV-1&2) in fingerstick blood at point-of-care sites by a rapid and sensitive lateral flow immunochromatographic assay.

Journal of immunological methods·2026
Same journal

Development of chimeric DGP-IgG antibodies as quality control for celiac disease diagnosis.

Journal of immunological methods·2026
Same journal

Stepwise single-cell-resolved deep immunophenotyping pipeline to characterise immune heterogeneity and functionality in health and disease.

Journal of immunological methods·2026
Same journal

Performance evaluation of Meso Scale Discovery (MSD) quantitative serological assays for detection of binding (IgG, IgA, IgM) and ACE2 inhibitory antibody levels for SARS-CoV-2.

Journal of immunological methods·2026
See all related articles

Related Experiment Video

Updated: Feb 14, 2026

Autofluorescence Imaging to Evaluate Cellular Metabolism
07:36

Autofluorescence Imaging to Evaluate Cellular Metabolism

Published on: November 15, 2021

5.3K

Autofluorescence: A potential pitfall in immunofluorescence-based inflammation grading.

Jonas Wizenty1, Muhammad Imtiaz Ashraf1, Nadine Rohwer2

  • 1Department of Surgery, Campus Charité Mitte and Campus Virchow Klinikum, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany.

Journal of Immunological Methods
|February 20, 2018
PubMed
Summary
This summary is machine-generated.

Autofluorescence (AF) in colon tissues can obscure immunofluorescence staining results, especially in inflamed tissues. This study identifies AF sources and demonstrates that Sudan Black B or copper sulfate can reduce AF, improving diagnostic accuracy.

Keywords:
AutofluorescenceCopper sulfateIBDIntestineLipofuscinSudan black b

More Related Videos

Classification of Neural Stem Cell Activation State In Vitro using Autofluorescence
06:56

Classification of Neural Stem Cell Activation State In Vitro using Autofluorescence

Published on: April 12, 2024

1.0K
Establishing a Severe Corneal Inflammation Model in Rats Based on Corneal Epithelium Curettage Combined with Corneal Sutures
04:48

Establishing a Severe Corneal Inflammation Model in Rats Based on Corneal Epithelium Curettage Combined with Corneal Sutures

Published on: November 22, 2024

890

Related Experiment Videos

Last Updated: Feb 14, 2026

Autofluorescence Imaging to Evaluate Cellular Metabolism
07:36

Autofluorescence Imaging to Evaluate Cellular Metabolism

Published on: November 15, 2021

5.3K
Classification of Neural Stem Cell Activation State In Vitro using Autofluorescence
06:56

Classification of Neural Stem Cell Activation State In Vitro using Autofluorescence

Published on: April 12, 2024

1.0K
Establishing a Severe Corneal Inflammation Model in Rats Based on Corneal Epithelium Curettage Combined with Corneal Sutures
04:48

Establishing a Severe Corneal Inflammation Model in Rats Based on Corneal Epithelium Curettage Combined with Corneal Sutures

Published on: November 22, 2024

890

Area of Science:

  • Cellular and Molecular Biology
  • Histopathology
  • Immunology

Background:

  • Immunofluorescence (IF) staining is crucial for research and diagnostics, but tissue autofluorescence (AF) can interfere.
  • AF is often overlooked, leading to inaccurate interpretations in IF assays.
  • Cellular AF is present in healthy and inflamed human and murine colonic tissues.

Purpose of the Study:

  • To characterize cellular AF in paraffin-embedded colonic tissues.
  • To investigate methods for reducing AF to improve IF staining.
  • To explore AF as a potential biomarker for colonic inflammation.

Main Methods:

  • Analysis of AF in human and murine colonic tissues using various excitation and emission spectra.
  • Identification of AF sources, including lipofuscin in immune cells and Paneth cell granules.
  • Application of Sudan Black B (SBB) and copper sulfate treatments to reduce AF.

Main Results:

  • AF signals were detected across a broad spectrum (425–738 nm emission, 403.6–638.7 nm excitation).
  • AF was more pronounced in inflamed tissues, primarily in subepithelial areas and blood vessels.
  • SBB and copper sulfate treatments significantly reduced AF signals.

Conclusions:

  • AF is an intrinsic biomarker of colonic inflammation.
  • AF predominantly occurs in immune cells within inflammatory bowel disease (IBD) tissues.
  • Effective AF reduction is critical for accurate IF interpretation and highlights limitations in diagnostic applications.