Hymenobacter profundi sp. nov., isolated from deep-sea water

A Gram-stain-negative, rod-shaped, red-pigmented, aerobic bacterium, strain M2^T, was isolated from a seawater sample collected from the western Pacific Ocean at a depth of 1000 m and characterized using polyphasic taxonomy. Strain M2^T was catalase-positive and oxidase-negative. Cells grew at 4-33 °C (optimum, 25 °C), at pH 6-9 (optimum, 7) and with 0-4 % (w/v) (optimum, 1 %) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain M2^T was associated with the genus Hymenobacter. Strain M2^T showed the highest 16S rRNA gene sequence similarities to Hymenobacter actinosclerus CCUG 39621^T (95.7 %), Hymenobacter tibetensis XTM003^T (95.6 %) and Hymenobacter psychrotolerans Tibet-IIU11^T (95.2 %). The DNA G+C content was 59.98 mol%. Strain M2^T contained C_16 : 1ω5c (25.0 %), iso-C_15 : 0 (23.9 %) and summed feature 3 (C_16 : 1ω6c and/or C_16 : 1ω7c, 20.4 %) as major cellular fatty acids. The major quinone of strain M2^T was menaquinone 7 and the major polar lipid was phosphatidylethanolamine. The major polyamine of strain M2^T was sym-homospermidine. The phylogenetic analysis and physiological and biochemical data showed that strain M2^T should be classified as representing a novel species of the genus Hymenobacter, for which the name Hymenobacter profundi sp. nov. is proposed. The type strain is M2^T (=CCTCC AB 2017185^T=KCTC 62120^T).