Related Experiment Video

Updated: Feb 14, 2026

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts
10:55

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts

Published on: November 5, 2012

17.1K

Oncoprotein Tudor-SN is a key determinant providing survival advantage under DNA damaging stress

Xiao Fu1, Chunyan Zhang1, Hao Meng1

  • 1Key Laboratory of Immune Microenvironment and Disease, Ministry of Education, Key Laboratory of Cellular and Molecular Immunology in Tianjin, Department of Biochemistry and Molecular Biology, Excellent Talent Project, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, 300070, Tianjin, China.

Cell Death and Differentiation
|February 21, 2018
PubMed

Insights

Tudor-SN protein is crucial for early DNA damage response by recruiting repair factors and promoting cell survival. Its dysfunction impairs DNA repair, suggesting a role in cancer radioresistance.

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • DNA damage response (DDR) is essential for maintaining genomic integrity.
  • Tudor-SN is a protein implicated in cellular processes, but its specific role in DDR is not fully understood.

Purpose of the Study:

  • To elucidate the function of Tudor-SN in the early stages of DNA damage response.
  • To investigate the molecular mechanisms by which Tudor-SN participates in DDR and cell survival.

Main Methods:

  • Utilized X-ray and laser irradiation to induce DNA damage in cellular models.
  • Investigated protein-protein interactions using techniques such as co-immunoprecipitation.
  • Assessed chromatin structure changes and DNA repair pathway activation via molecular assays.

Main Results:

  • Tudor-SN accumulates at DNA damage sites in a poly(ADP-ribosyl)ation-dependent manner, interacting with PARP-1 via its SN domain.
  • Tudor-SN recruits SMARCA5 and GCN5 to damage sites, leading to chromatin relaxation and ATM kinase activation.
  • Loss-of-function of Tudor-SN resulted in impaired chromatin relaxation, reduced recruitment of repair factors, and deficiencies in DNA repair and cell survival.

Conclusions:

  • Tudor-SN is a key regulator of the early DNA damage response, orchestrating chromatin remodeling and activating repair pathways.
  • Tudor-SN's role in promoting cell survival after DNA damage highlights its potential involvement in cancer radioresistance due to its high expression in tumors.

More Related Videos

Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay10:00

Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay

Published on: December 19, 2012

28.2K
A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage07:57

A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage

Published on: May 10, 2022

6.0K

Related Concept Videos

DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.2K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.2K
Key Elements for Plant Nutrition02:35

Key Elements for Plant Nutrition

Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
24.4K
Key Techniques in Microbiology01:19

Key Techniques in Microbiology

Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...
2.5K
Survival Curves01:18

Survival Curves

Survival curves are graphical representations that depict the survival experience of a population over time, offering an intuitive way to track the proportion of individuals who remain event-free at each time point. These curves are widely used in fields such as medicine, public health, and reliability engineering to visualize and compare survival probabilities across different groups or conditions.
The Kaplan-Meier estimator is the most common method for constructing survival curves. This...
745
Survival Tree01:19

Survival Tree

Survival trees are a non-parametric method used in survival analysis to model the relationship between a set of covariates and the time until an event of interest occurs, often referred to as the "time-to-event" or "survival time." This method is particularly useful when dealing with censored data, where the event has not occurred for some individuals by the end of the study period, or when the exact time of the event is unknown.
 Building a Survival Tree
Constructing a...
437