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Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
Published on: February 18, 2014
Silver-mediated direct C-H amination of BODIPYs for screening endoplasmic reticulum-targeting reagents
Huaxing Zhang1, Xingyu Chen, Jingbo Lan
1Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, P. R. China. jingbolan@scu.edu.cn wood@scu.edu.cn jsyou@scu.edu.cn.
Abstract:
A highly efficient direct C-H amination of BODIPYs has been accomplished through the Ag(i)-mediated nucleophilic addition of an amino radical to BODIPY at the C3- and/or C5-position and deprotonation processes under mild conditions. This protocol greatly streamlines the access to a variety of 3-aminated and 3,5-diaminated BODIPYs. The resulting BODIPYs with morpholine groups (3q and 4b) exhibit specific endoplasmic reticulum (ER)-localization capacities and negligible cytotoxicities, which would be potential ER-targeting reagents.
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