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[Quality control study of engineered skin tissue].
K V Nakhod1, A L Rusanov2, E D Luzgina2
1Institute of Biomedical Chemistry, Moscow, Russia.
Biomeditsinskaia Khimiia
|February 21, 2018
Summary
OMERO service facilitated expert annotation of HaCaT cell images for tissue engineering. It proved useful for quality control in cell preparation development and standardization.
Area of Science:
- Biotechnology
- Cell Biology
- Microscopy
Background:
- Developing tissue-engineered epithelium requires quality control of keratinocyte cell preparations.
- Standardized annotation and cross-validation are crucial for reliable cell culture development.
Purpose of the Study:
- To evaluate the utility of the OMERO service for annotating microscope images of HaCaT cells.
- To assess the reliability of expert opinions through quantitative metrics like accuracy, sensitivity, and specificity.
- To demonstrate OMERO's role in dynamic cross-validation for cell preparation standardization.
Main Methods:
- OMERO service was employed for image annotation by two independent expert groups.
- Microscope images of HaCaT cell line, used in tissue-engineered epithelium development, were annotated.
- Expert opinions were evaluated using specificity, sensitivity, and accuracy calculations.
Main Results:
- The highest agreement (91%) between expert groups was observed for cell monolayer confluence.
- An accuracy of 70% was achieved in determining cell differentiation after 10 days.
- OMERO service enabled dynamic cross-validation of image annotation quality.
Conclusions:
- OMERO service is a valuable tool for annotating and quality-controlling cell preparations.
- The study highlights OMERO's effectiveness in standardizing cell-based assays and tissue engineering.
- Quantitative evaluation of expert annotations confirms OMERO's utility in research settings.
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