Functional characterization of the citrus canker susceptibility gene CsLOB1
Shuo Duan1,2, Hongge Jia2, Zhiqian Pang2
1Citrus Research Institute, Southwest University, Chongqing, 400712, China.
Molecular Plant Pathology
|February 21, 2018
Summary
Xanthomonas citri ssp. citri (Xcc) causes citrus canker by manipulating the CsLOB1 gene. This study reveals CsLOB1’s nuclear function and identifies its direct target, advancing understanding of citrus canker development.
Area of Science:
- Plant Pathology
- Molecular Biology
- Genetics
Background:
- Citrus canker, caused by Xanthomonas citri ssp. citri (Xcc), is a devastating global disease.
- The Xcc transcriptional activator-like (TAL) effector PthA is known to regulate the citrus susceptibility gene CsLOB1.
Purpose of the Study:
- To functionally characterize the CsLOB1 gene and its role in citrus canker pathogenesis.
- To identify direct molecular targets of CsLOB1.
Main Methods:
- Subcellular localization of CsLOB1 protein in citrus protoplasts.
- Induction of CsLOB1 expression in CsLOB1-GR transgenic plants using dexamethasone (DEX).
- Bioinformatic analysis and electrophoretic mobility shift assays (EMSAs) to identify CsLOB1 targets.
Main Results:
- CsLOB1 protein was localized to the nucleus in citrus cells.
- DEX-induced CsLOB1 expression in transgenic plants led to pustule formation and water soaking, mimicking citrus canker symptoms.
- CsLOB1 directly targets Cs2g20600, a gene encoding a zinc finger C3HC4-type RING finger protein.
Conclusions:
- CsLOB1 functions as a nuclear transcriptional factor crucial for citrus canker symptom development.
- The study elucidates a key molecular mechanism by which Xcc manipulates host susceptibility genes to cause disease.
- Identification of Cs2g20600 as a direct CsLOB1 target provides new insights into the downstream regulatory network of citrus canker.
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