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Autofluorescence-Free Live-Cell Imaging Using Terbium Nanoparticles.

M Cardoso Dos Santos1, J Goetz2, H Bartenlian1

  • 1NanoBioPhotonics (nanofret.com), Institute for Integrative Biology of the Cell (I2BC) , Université Paris-Saclay, Univeristé Paris-Sud, CNRS, CEA , 91405 Orsay Cedex, France.

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Summary
This summary is machine-generated.

New terbium nanoparticles (Tb-NPs) offer bright, autofluorescence-free intracellular imaging. These long-lifetime nanoparticles enable sensitive, time-gated imaging of live cells for extended periods without toxicity.

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Area of Science:

  • Nanotechnology
  • Biomedical Imaging
  • Cell Biology

Background:

  • Fluorescent nanoparticles (NPs) are crucial for cellular imaging.
  • UV/visible light excitation causes autofluorescence, while NIR-excitable NPs lack brightness.
  • A need exists for bright, autofluorescence-free imaging agents.

Purpose of the Study:

  • To introduce surface-photosensitized terbium nanoparticles (Tb-NPs) for autofluorescence-free intracellular imaging.
  • To evaluate the performance of Tb-NPs in live HeLa cells.
  • To demonstrate the potential of Tb-NPs for advanced live-cell imaging.

Main Methods:

  • Synthesis and characterization of surface-photosensitized terbium nanoparticles (Tb-NPs).
  • Intracellular imaging experiments in live HeLa cells using time-gated photoluminescence (PL).
  • Assessment of NP concentration, imaging duration, toxicity, and PL decay curves.

Main Results:

  • Tb-NPs demonstrated exceptionally high brightness and photostability.
  • Autofluorescence-free intracellular imaging of vesicles was achieved over 72 hours.
  • Effective time-gated PL imaging was possible at concentrations as low as 12 pM without toxicity.
  • Detection of long-lifetime (ms) PL decay curves from small cellular areas was achieved rapidly.

Conclusions:

  • Tb-NPs possess unprecedented photophysical properties for live-cell imaging.
  • These nanoparticles overcome limitations of current imaging agents, offering superior performance.
  • Tb-NPs represent a significant advancement for high-resolution, long-term intracellular imaging.