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Updated: Feb 14, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Selective extraction of lipase and amylase from enzyme mixture by employing liquid emulsion membrane
B S Priyanka1, Navin K Rastogi1
1Dept. of Food Engineering, Academy of Scientific and Innovative Research, CSIR-Central Food Technological Research Institute, Mysore, Karnataka, 570020, India.
Abstract:
This work deals with the extraction of lipase and amylase from enzyme mixture by employing liquid emulsion membranes (LEM). The electrostatic interaction between enzymes and reverse micellar surfactant polar head group plays an important role for selective extraction of two different enzymes having different isoelectric points. The optimized conditions for lipase extraction (pH 7.0) resulted in the purification fold and activity recovery of 5.43 fold and 89.53%, respectively, whereas, in case of amylase (pH 9.0) the purification fold and activity recovery were 6.58 and 94.32%, respectively. The results were compared with the control sample (containing individual enzymes) and mixture of enzymes lipase and amylase and it was shown that for optimum conditions the activity recovery and purification fold was higher for the individual enzymes as compared to their mixture. Downstream processing involving LEM was shown to be a feasible method for selective extraction of enzymes. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:721-729, 2018.
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