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Related Concept Videos

Glucose Transporters01:27

Glucose Transporters

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Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
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The Colonization of Land02:22

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Changes in the environment of the early Earth drove the evolution of organisms. As prokaryotic organisms in the oceans began to photosynthesize, they produced oxygen. Eventually, oxygen saturated the oceans and entered the air, resulting in an increase in atmospheric oxygen concentration, known as the oxygen revolution approximately 2.3 billion years ago. Therefore, organisms that could use oxygen for cellular respiration had an advantage. More than 1.5 years ago, eukaryotic cells and...
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Secondary Active Transport01:55

Secondary Active Transport

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One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme “pump” embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
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Glucose Homeostasis: Regulation of Blood Glucose01:02

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Carbohydrates consumed through foods are converted into glucose, a crucial energy source for the body. In the prandial state, high blood glucose levels stimulate the secretion of insulin from the pancreas. Insulin inhibits hepatic glucose production and stimulates glucose uptake and metabolism by muscle and adipose tissue. The excess glucose is converted into glycogen and stored in the liver and muscles.
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
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Facilitated Transport01:19

Facilitated Transport

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The chemical and physical properties of plasma membranes cause them to be selectively permeable. Since plasma membranes have both hydrophobic and hydrophilic regions, substances need to be able to transverse both regions. The hydrophobic area of membranes repels substances such as charged ions. Therefore, such substances need special membrane proteins to cross a membrane successfully. In  facilitated transport, also known as facilitated diffusion, molecules and ions travel across a...
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Cell Specific Gene Expression01:58

Cell Specific Gene Expression

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Related Experiment Video

Updated: Feb 14, 2026

A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood
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Glucose transporter expression in the human colon.

Flavia Merigo1, Alessandro Brandolese2, Sonia Facchin3

  • 1Department of Neuroscience, Biomedicine and Movement, Human Anatomy and Histology Section, University of Verona, Verona I-37134, Italy. flavia.merigo@univr.it.

World Journal of Gastroenterology
|February 23, 2018
PubMed
Summary

Glucose transporter expression, including GLUT5, was found in colorectal mucosa of both healthy individuals and those with inflammatory bowel disease (IBD). Notably, GLUT5 is linked to lymphatic vessels in the gut.

Keywords:
ColonCrohn’s diseaseGlucose transporterLYVE-1, ImmunohistochemistryUlcerative colitis

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Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Immunohistochemistry

Background:

  • Inflammatory Bowel Disease (IBD) involves chronic inflammation of the gastrointestinal tract.
  • Glucose transporters play crucial roles in cellular metabolism and nutrient absorption.
  • Understanding glucose transporter expression in IBD is vital for characterizing disease mechanisms.

Purpose of the Study:

  • To investigate glucose transporter (GLUT2, SGLT1, GLUT5) expression in human colorectal mucosa.
  • To compare transporter expression between healthy controls and IBD patients.
  • To explore the association of glucose transporters with lymphatic vasculature in the colorectum.

Main Methods:

  • Immunohistochemistry was used to analyze 147 colorectal biopsies from IBD patients (ulcerative colitis, Crohn's disease) and controls.
  • Expression levels of GLUT2, SGLT1, and GLUT5 were assessed.
  • Double-labeling with LYVE-1 (lymphatic marker) and GLUT5 was performed using confocal microscopy.

Main Results:

  • GLUT2, SGLT1, and GLUT5 were expressed in the colonic epithelium, with no significant differences between groups or colorectal regions.
  • Unexpectedly, GLUT5 expression was detected in colorectal vessels.
  • GLUT5-positive vessels, identified as lymphatic vessels via LYVE-1 co-staining, were observed in both IBD and control tissues.

Conclusions:

  • Glucose transporters are expressed in colorectal mucosa in both healthy individuals and IBD patients.
  • GLUT5 expression is associated with lymphatic vessels in the colon.
  • This finding contributes to the understanding of lymphatic vasculature in the context of IBD.