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Updated: Feb 14, 2026

Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies
Published on: May 19, 2023
[Preparation of anti-SEA egg yolk immunoglobulin and its preliminary application]
Wu Kai1, Xu Ming-Xing1, Yang Yan1
1Department of Schistosomiasis and Endemic Diseases, Wuhan Center for Disease Control and Prevention, Hubei Province, Wuhan 430015, China.
Objective:
To prepare the egg yolk immunoglobulin (IgY) against soluble egg antigen (SEA) of Schistosoma japonicum, and explore its feasibility for schistosomiasis screening in endemic areas.
Methods:
The urine samples of indirect haemagglutination test (IHA) positives from endemic areas and healthy people from non-endemic areas were collected. The Anti-SEA IgY was obtained through immunization by subcutaneous injection of SEA into Laihang hen, and its molecular weight was determined by SDS polyacrylamide gel electrophoresis (SDS-PAGE) . The circulating soluble egg antigen (CSEA) in the urine of IHA positives and healthy people were detected by ELISA-double antibody sandwich method with the Anti-SEA IgY as the capture antibody.
Results:
Anti-SEA IgY was prepared and purified successfully. Totally 48 urine samples of IHA positives were detected by ELISA and 26 of them were CSEA positives (54.17%); 10 urine samples of healthy people were detected, and all of them were negative.
Conclusions:
The CSEA in human urine can be detected effectively by ELISA based on IgY. As a convenient and atraumatic method, it could be applied in schistosomiasis screening.
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