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Metabolomics in Plant Stress Physiology
Arindam Ghatak1, Palak Chaturvedi1, Wolfram Weckwerth2,3
1Department of Ecogenomics and Systems Biology, Faculty of Sciences, University of Vienna, Vienna, Austria.
Advances in Biochemical Engineering/Biotechnology
|February 23, 2018
Summary
Plant metabolomics uses techniques like NMR, CE-MS, LC-MS, and GC-MS to study gene functions and stress responses. This research identifies key metabolite markers and processes involved in plant stress adaptation.
Area of Science:
- Plant science
- Systems biology
- Functional genomics
Background:
- Metabolomics is crucial for understanding gene function and biological systems.
- It aids in analyzing plant responses to various environmental stresses.
- Various analytical techniques are employed to capture the full metabolome.
Purpose of the Study:
- To provide an overview of plant metabolomic techniques.
- To discuss data analysis, compound identification, and limitations.
- To summarize stress-responsive metabolite markers and processes.
Main Methods:
- Nuclear Magnetic Resonance (NMR)
- Capillary Electrophoresis-Mass Spectrometry (CE-MS)
- Liquid Chromatography-Mass Spectrometry (LC-MS)
- Gas Chromatography-Mass Spectrometry (GC-MS)
Main Results:
- The chapter details the workflow for measuring plant metabolites.
- It highlights the importance of technique selection based on speed, sensitivity, and accuracy.
- A broad range of stress-responsive metabolite markers and processes are compiled.
Conclusions:
- Metabolomics is a vital tool for plant functional genomics and systems biology.
- Understanding plant stress responses through metabolomics is a significant global research area.
- The study provides a comprehensive overview of plant metabolomics for stress research.
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