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05:52
Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
Published on: September 27, 2019
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Innovative Cryopreservation Process Using a Modified Core/Shell Cell-Printing with a Microfluidic System for
Jae Yoon Lee1, YoungWon Koo1, GeunHyung Kim1
1Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering , Sungkyunkwan University (SKKU) , Suwon 16419 , South Korea.
ACS Applied Materials & Interfaces
|February 24, 2018
Summary
This study presents a novel core/shell cell-printing method for creating 3D collagen scaffolds. These scaffolds enable medium-term cryopreservation of cells with high viability for tissue engineering applications.
Area of Science:
- Biomaterials Engineering
- Tissue Engineering
- Cell Biology
Background:
- Cryopreservation of cell-laden scaffolds is crucial for tissue engineering.
- Maintaining cell viability and function post-cryopreservation remains a challenge.
- 3D printing offers precise control over scaffold architecture for cell encapsulation.
Purpose of the Study:
- To investigate the printability and applicability of a core/shell cell-printed scaffold for medium-term cryopreservation.
- To develop an innovative cell-printing process for cryopreservable 3D porous collagen scaffolds.
- To assess cell viability, growth, and function after cryopreservation and subsequent cultivation.
Main Methods:
- Development of a cell-printing process using a microfluidic channel, core/shell nozzle, and low-temperature stage.
- Fabrication of 3D porous collagen scaffolds with core/shell struts (collagen bioink/DMSO core, alginate/poly(ethylene oxide) shell).
- Cryopreservation of cell-laden scaffolds for 2 weeks, followed by cultivation of osteoblast-like cells or human adipose stem cells.
Main Results:
- The core/shell cell-printed scaffolds demonstrated successful printability and cryopreservation.
- Cells within the scaffold exhibited high viability (over 90%) after 2 weeks of cryopreservation.
- Post-cryopreservation cultivation showed steady cell growth and mineralization comparable to non-cryopreserved controls.
Conclusions:
- The developed fabrication process enables the creation of cryopreservable core/shell cell-laden collagen scaffolds.
- Optimized freezing and thawing processes contribute to excellent cell survival and function.
- This technology holds significant potential for various tissue engineering applications requiring cell banking and storage.
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