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X-Ray Fluorescence-Detected Flow Cytometry.

Andrew M Crawford1, James E Penner-Hahn2

  • 1Department of Geology, University of Saskatchewan, Saskatoon, SK, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|February 25, 2018
PubMed
Summary
This summary is machine-generated.

X-ray fluorescence-detected flow cytometry analyzes cellular elements using synchrotron X-rays. This developing technique characterizes elemental variability in various cell types, offering new insights into cell composition.

Keywords:
CellsCytometerFlow cytometerMetallomeX-ray fluorescence

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Area of Science:

  • Analytical Chemistry
  • Cell Biology
  • Biophysics

Background:

  • Elemental analysis at the cellular level is crucial for understanding biological processes.
  • Existing techniques face limitations in sensitivity and cellular resolution.
  • Synchrotron-based X-ray fluorescence offers high sensitivity for elemental detection.

Purpose of the Study:

  • To describe the experimental setup for X-ray fluorescence-detected flow cytometry.
  • To detail the data collection and processing methodologies for this technique.
  • To highlight its potential for characterizing cellular elemental content.

Main Methods:

  • Utilizing synchrotron-induced X-ray emission for elemental analysis.
  • Applying flow cytometry principles for single-cell analysis.
  • Analyzing fully aquated, actively respiring cells to maintain physiological conditions.

Main Results:

  • Demonstrated the capability to detect and characterize ultra-trace, trace, and bulk elemental content in cells.
  • Successfully applied the technique to bovine red blood cells, Saccharomyces cerevisiae, and NIH3T3 mouse fibroblasts.
  • Provided a foundation for understanding cell-to-cell elemental variability.

Conclusions:

  • X-ray fluorescence-detected flow cytometry is a promising, albeit developing, technique for cellular elemental analysis.
  • The described methods enable detailed characterization of elemental composition at the single-cell level.
  • This approach opens new avenues for research in cell biology and elemental toxicology.