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Related Experiment Video

Updated: Feb 14, 2026

Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy

Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy

Published on: January 7, 2012

Kidney Imaging: Intravital Microscopy.

Takashi Hato¹, Seth Winfree¹, Pierre C Dagher^2,3

¹Department of Medicine, Indiana University, Indianapolis, IN, USA.

Methods in Molecular Biology (Clifton, N.J.)

|February 25, 2018

Summary

This summary is machine-generated.

Intravital two-photon microscopy enables live animal imaging. This chapter details rodent kidney imaging techniques, including surgery, autofluorescence, probes, and immune cell tracking.

Keywords:

Intravital two-photon microscopy Kidney Oxidative stress Renal immune cell imaging

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Area of Science:

* In vivo imaging
* Renal biology
* Microscopy

Background:

* Intravital two-photon microscopy is crucial for studying biological processes in live animals.
* Understanding kidney function requires advanced imaging techniques.
* Rodent models are frequently used in renal research.

Purpose of the Study:

* To provide an overview of intravital imaging techniques for the rodent kidney.
* To describe essential methods for successful kidney imaging in vivo.
* To highlight applications in renal research, such as immune cell tracking.

Main Methods:

* Detailed surgical procedures for rodent kidney preparation.
* Characterization of intrinsic renal tubular autofluorescence.
* Application and considerations for in vivo fluorescent probes.
* Techniques for tracking renal immune cells in real-time.

Main Results:

* Successful application of intravital two-photon microscopy to the rodent kidney.
* Identification of autofluorescence properties aiding imaging.
* Demonstration of fluorescent probe utility for specific cell and structure visualization.
* Effective tracking of immune cell dynamics within the kidney.

Conclusions:

* Intravital two-photon microscopy is a valuable tool for rodent kidney research.
* The described methods facilitate detailed investigation of renal physiology and pathology.
* This imaging approach supports advancements in understanding kidney immune responses and disease mechanisms.