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DNA in psoriatic epidermis.

J Sondergaard, A Lerche, V Bohr

    Acta Dermato-Venereologica. Supplementum
    |January 1, 1979
    PubMed
    Summary
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    Psoriasis patients treated with 8-methoxypsoralen (8-MOP) and UV light (PUVA) showed no significant DNA damage. Electron microscopy revealed DNA crosslink frequencies similar to normal skin, regardless of topical or systemic treatment.

    Area of Science:

    • Dermatology
    • Molecular Biology
    • Photochemistry

    Background:

    • Psoriasis treatment often involves 8-methoxypsoralen (8-MOP) combined with ultraviolet A (UVA) light (PUVA therapy).
    • Concerns exist regarding potential DNA damage from PUVA treatment.
    • Previous in vitro studies demonstrated 8-MOP's ability to induce DNA crosslinks upon UVA irradiation.

    Purpose of the Study:

    • To investigate and quantify DNA interstrand crosslinks in skin cells of psoriasis patients undergoing PUVA treatment.
    • To compare DNA crosslink frequency in epidermal and dermal cells.
    • To assess if PUVA treatment leads to significantly increased genetic damage compared to normal skin.

    Main Methods:

    • Electron microscopy was employed to visualize and measure DNA crosslinks after total denaturation.

    Related Experiment Videos

  • DNA was isolated from 30 skin biopsies of 14 psoriasis patients (topical and systemic PUVA treatment).
  • A total of 9503 DNA molecules were analyzed for crosslink presence and density.

    • Main Results:

    • The frequency of DNA molecules with three or more crosslinks was 1%.
    • Overall crosslink frequency was similar in epidermis (1.1%) and dermis (0.9%).
    • DNA crosslink levels were comparable between topical and systemic PUVA treatments and similar to normal human skin.

    Conclusions:

    • PUVA treatment, using 8-MOP and UVA, did not result in significantly increased DNA damage in psoriasis patients.
    • The observed DNA crosslink frequencies were consistent across different skin layers and treatment modalities.
    • Findings suggest PUVA therapy's DNA crosslinking effects in vivo are comparable to those in normal skin.