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Isolation of Cellular Lipid Droplets: Two Purification Techniques Starting from Yeast Cells and Human Placentas
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Meso-Endothelial Bipotent Progenitors from Human Placenta Display Distinct Molecular and Cellular Identity.
Abbas Shafiee1, Jatin Patel2, Dietmar W Hutmacher3
1UQ Centre for Clinical Research, The University of Queensland, Brisbane, QLD, Australia; Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, QLD, Australia; UQ Diamantina Institute, Translational Research Institute, The University of Queensland, Brisbane, QLD, Australia.
Stem Cell Reports
|February 27, 2018
Summary
Human placenta contains bipotential progenitor cells that can develop into both endothelial and mesenchymal cells. These cells, identified via specific markers, are crucial for understanding cell differentiation and tissue development.
Area of Science:
- Stem Cell Biology
- Developmental Biology
- Human Placenta Research
Background:
- The existence of bipotential stem/progenitor cells capable of differentiating into both mesenchymal and endothelial lineages in human postnatal tissues remains debated.
- Investigating such cells is crucial for understanding tissue regeneration and development.
Purpose of the Study:
- To investigate the presence and characteristics of bipotential progenitor cells within the human term placenta.
- To identify the specific cell population and its differentiation potential ex vivo.
Main Methods:
- Utilized flow cytometry to isolate a specific cell population (CD45-CD34+CD144+CD31Lo) from human term placenta.
- Performed limiting dilution culture assays to assess the differentiation capacity of isolated cells into endothelial and mesenchymal lineages.
- Conducted RNA sequencing and functional analyses to explore the molecular mechanisms driving progenitor function.
Main Results:
- A unique bipotential progenitor cell population (CD45-CD34+CD144+CD31Lo) was successfully isolated from the human term placenta.
- These bipotential cells demonstrated the capacity to differentiate into both endothelial and mesenchymal colonies in vitro.
- Notch signaling was identified as a key pathway regulating endothelial and bipotential progenitor function, while TGFβ receptor inhibition did not impact mesenchymal cell formation.
Conclusions:
- The human term placenta harbors bipotential progenitor cells with the capacity to generate both endothelial and mesenchymal cells.
- These findings reveal novel insights into the cellular and molecular mechanisms governing progenitor cell differentiation in the placenta.
- The identified bipotential progenitors may play a significant role in placental development and function.
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