Two flavonol glycosides from Liparis bootanensis

Area of Science:

• Natural Product Chemistry
• Pharmacology
• Medicinal Chemistry

Background:

• Liparis bootanensis Griff. is a plant species with potential medicinal properties.
• Flavonol glycosides are a class of natural compounds known for various biological activities.
• Understanding the chemical constituents and bioactivities of traditional medicinal plants is crucial for drug discovery.

Purpose of the Study:

• To isolate and characterize new compounds from Liparis bootanensis Griff.
• To evaluate the cytotoxic and anti-inflammatory activities of the isolated compounds.
• To explore the potential of Liparis bootanensis Griff. as a source of therapeutic agents.

Main Methods:

• Isolation of compounds using chromatographic techniques.
• Structure elucidation using high-resolution electrospray-ionization mass spectrometry (HR-ESIMS) and nuclear magnetic resonance (NMR) spectroscopy (1D and 2D).
• Cytotoxicity assays against HCT116 human cancer cell line.
• In vitro anti-inflammatory assays using lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells to measure inducible nitric oxide synthase (iNOS) protein expression.

Main Results:

• Two new flavonol glycosides, bootanenside I and II, were isolated along with ten known compounds.
• None of the isolated compounds exhibited significant cytotoxicity against HCT116 cancer cells.
• Compounds bootanenside I and II demonstrated moderate in vitro anti-inflammatory activity by inhibiting iNOS protein expression in LPS-stimulated macrophages.

Conclusions:

• Liparis bootanensis Griff. contains novel flavonol glycosides with potential anti-inflammatory properties.
• The anti-inflammatory activity is mediated through the inhibition of inducible nitric oxide synthase (iNOS).
• Further research is warranted to explore the therapeutic potential of these compounds and their mechanism of action.