Improved Isotopic Profiling by Pure Shift Heteronuclear 2D J-Resolved NMR Spectroscopy

Davy Sinnaeve1, Mickael Dinclaux2, Edern Cahoreau2

  • 1NMR and Structure Analysis Unit, Department of Organic and Macromolecular Chemistry , Ghent University , Ghent , B-9000 , Belgium.

Analytical Chemistry
|February 27, 2018
PubMed
Summary

Pure shift 2D J-resolved NMR spectroscopy simplifies metabolite analysis. This method extracts crucial carbon isotope data from bacterial cell lysates, aiding metabolic flux analysis.

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