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Methotrexate Reduces DNA Integrity in Sperm From Men With Inflammatory Bowel Disease
Dana Ley1, Jeffrey Jones2, John Parrish3
1University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
Gastroenterology
|February 27, 2018
Summary
Methotrexate exposure in men with inflammatory bowel diseases (IBDs) may reduce sperm integrity. This study found increased DNA fragmentation and oxidative stress in sperm from IBD patients on methotrexate compared to controls.
Area of Science:
- Reproductive medicine
- Immunology
- Toxicology
Background:
- Limited data exist on methotrexate's impact on male reproductive capacity, particularly in inflammatory bowel disease (IBD) patients.
- Methotrexate is a common immunosuppressant used for IBD treatment.
Purpose of the Study:
- To investigate the effects of methotrexate on sperm quality and genetic integrity in men with IBD.
- To compare sperm parameters between IBD patients exposed to methotrexate and a control group.
Main Methods:
- A case-control study comparing 7 men with IBD exposed to methotrexate for ≥3 months with 1912 age-matched men from fertility centers.
- Sperm evaluation included basic semen analysis and advanced sperm integrity testing (DNA fragmentation, oxidative stress markers).
Main Results:
- Basic semen analysis parameters were normal in IBD patients on methotrexate.
- Sperm samples from IBD patients showed significantly reduced integrity, with increased DNA fragmentation and oxidative stress damage compared to controls.
Conclusions:
- Methotrexate exposure in men with IBD is associated with reduced sperm DNA integrity.
- Methotrexate may induce sperm damage through oxidative stress pathways in this patient population.
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