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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
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Sensitive and selective Affimer-functionalised interdigitated electrode-based capacitive biosensor for Her4 protein
Pavel Zhurauski1, Sunil K Arya1, Pawan Jolly1
1Department of Electronic and Electrical Engineering, University of Bath, Bath BA2 7AY, United Kingdom.
Biosensors & Bioelectronics
|February 27, 2018
Summary
A novel capacitive biosensor using Affimer technology can detect the Her4 tumor biomarker in undiluted serum with high sensitivity and specificity. This Affimer sensor shows potential for developing new diagnostic tools.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Analytical Chemistry
Background:
- Her4 is a protein tumor biomarker.
- Current detection methods may lack sensitivity or require sample dilution.
- Affimer technology offers a potential alternative for biomarker detection.
Purpose of the Study:
- To develop a novel Affimer-functionalised interdigitated electrode-based capacitive biosensor.
- To detect and quantify the Her4 protein biomarker in undiluted serum.
- To evaluate the sensor's sensitivity, specificity, and dynamic range.
Main Methods:
- Fabrication of gold interdigitated electrodes functionalised with anti-Her4 Affimers via self-assembly.
- Utilisation of Electrochemical Impedance Spectroscopy (EIS) to monitor capacitance changes for detection.
- Testing sensor performance in buffer and undiluted serum.
Main Results:
- The Affimer sensor demonstrated high sensitivity with a limit of detection lower than 1 pM.
- A broad dynamic range from 1 pM to 100 nM was observed in both buffer and serum.
- Excellent specificity was shown, with negligible interference from other serum proteins.
Conclusions:
- The developed Affimer-based capacitive biosensor is effective for detecting Her4 in undiluted serum.
- The sensor exhibits high sensitivity, specificity, and resilience to non-specific binding.
- This platform holds potential for adaptation to detect other biomarkers, paving the way for new biosensor development.
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