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P66Shc expression in diabetic rat retina.
Ming-Hui Zhao1,2, Jianyan Hu2, Shufeng Li2
1Department of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China.
BMC Ophthalmology
|February 28, 2018
Summary
P66Shc protein is present in rat retinas and increases with diabetes. Higher levels correlate with increased retinal cell apoptosis, suggesting a role in diabetic retinopathy development.
Area of Science:
- Biochemistry
- Cell Biology
- Ophthalmology
Background:
- P66Shc protein is linked to mitochondrial reactive oxygen species and apoptosis.
- P66Shc's role in the retina and diabetic retinopathy is unexplored.
- This study investigates P66Shc in streptozotocin-induced diabetic rat retinas.
Purpose of the Study:
- To investigate P66Shc expression in the retina of diabetic rats.
- To explore the potential role of P66Shc in diabetic retinopathy pathogenesis.
Main Methods:
- Detected P66Shc mRNA and protein using RT-PCR and Western blot in diabetic rats.
- Used immunohistochemistry to determine P66Shc localization in retinal tissues.
- Quantified retinal cell apoptosis via TUNEL assay.
Main Results:
- P66Shc mRNA and protein were detected in normal and diabetic rat retinas.
- Retinal P66Shc expression levels increased with diabetes progression.
- P66Shc was primarily located in the retinal ganglion cell and inner nuclear layers.
- Apoptosis rates significantly increased in diabetic rat retinas.
Conclusions:
- Rat retinal P66Shc expression is localized to the ganglion cell and inner nuclear layers.
- Increased P66Shc expression correlates with diabetes progression and elevated apoptosis.
- P66Shc may play a significant role in the development of diabetic retinopathy.
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