Related Experiment Video

Updated: Feb 13, 2026

Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood
07:26

Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood

Published on: September 14, 2017

11.9K

Generation of an anti-angiogenic endothelial progenitor cell line via endostatin gene transfer

Jing Ai1, Jun-Hui Sun2, Ting Wan1

  • 1Eye Centre, The Second Affiliated Hospital of The School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China.

Molecular Medicine Reports
|February 28, 2018
PubMed

Insights

Genetically engineered endothelial progenitor cells (EPCs) overexpressing endostatin show potential for treating ocular neovascularization (NV). This modification reduces vascular endothelial growth factor (VEGF) expression, offering an anti-angiogenic therapy.

Area of Science:

  • Regenerative Medicine
  • Molecular Biology
  • Ophthalmology

Background:

  • Endothelial progenitor cells (EPCs) are investigated for therapeutic neovascularization (NV).
  • Endostatin inhibits angiogenesis and suppresses vascular leakage.
  • Ocular NV requires novel anti-angiogenic therapies.

Purpose of the Study:

  • To create transgenic EPCs with anti-angiogenic properties for ocular NV treatment.
  • To genetically modify EPCs to overexpress endostatin.
  • To evaluate the anti-angiogenic effect of endostatin-transfected EPCs.

Main Methods:

  • EPCs isolated from rat peripheral blood.
  • Lentiviral vector used for endostatin and green fluorescent protein gene transfer into EPCs.
  • Reverse transcription-quantitative polymerase chain reaction and Western blot assays used to confirm gene and protein expression, including VEGF levels.

Main Results:

  • Stable endostatin-transfected EPC line generated after puromycin selection.
  • Increased endostatin mRNA and protein expression observed in transfected EPCs.
  • Decreased vascular endothelial growth factor (VEGF) expression confirmed in endostatin-transfected EPCs.

Conclusions:

  • EPCs can be successfully genetically modified to overexpress endostatin.
  • Endostatin overexpression in EPCs confers an anti-angiogenic effect by modulating VEGF.
  • Genetically modified EPCs represent a potential therapeutic strategy for ocular NV.
Keywords:
endostatingene transferendothelial progenitor cellslentiviral vector

More Related Videos

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells11:16

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

Published on: February 15, 2019

8.1K
Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing09:59

Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing

Published on: October 11, 2021

3.8K

Related Concept Videos

Horizontal Gene Transfer01:27

Horizontal Gene Transfer

Horizontal gene transfer (HGT) is a process where genetic material moves between organisms within the same generation, unlike vertical gene transfer, which occurs from parent to offspring. HGT plays a crucial role in microbial evolution, adaptation, and survival, particularly in shared environments like the human gut.Mobile genetic elements such as plasmids, prophages, integrons, insertion sequences, and transposons facilitate this process. HGT occurs through three primary mechanisms:...
2.3K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
Types of Genetic Transfer Between Organisms02:18

Types of Genetic Transfer Between Organisms

Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
31.1K
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
4.1K
Organization of Genes02:07

Organization of Genes

Overview
73.7K
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
13.4K