Related Experiment Video
Updated: Feb 13, 2026

Atomic Force Microscopy to Study the Physical Properties of Epidermal Cells of Live Arabidopsis Roots
Published on: March 31, 2022
The effect of root canal preparation on the surface roughness of WaveOne and WaveOne Gold files: atomic force
Taha Özyürek1, Koray Yılmaz2, Gülşah Uslu3
1Department of Endodontics, Ondokuz Mayis University Faculty of Dentistry, Samsun, Turkey.
Objectives:
To examine the surface topography of intact WaveOne (WO; Dentsply Sirona Endodontics) and WaveOne Gold (WOG; Dentsply Sirona Endodontics) nickel-titanium rotary files and to evaluate the presence of alterations to the surface topography after root canal preparations of severely curved root canals in molar teeth.
Materials And Methods:
Forty-eight severely curved canals of extracted molar teeth were divided into 2 groups (n = 24/each group). In group 1, the canals were prepared using WO and in group 2, the canals were prepared using WOG files. After the preparation of 3 root canals, instruments were subjected to atomic force microscopy analysis. Average roughness and root mean square values were chosen to investigate the surface features of endodontic files. The data was analyzed using one-way analysis of variance and post hoc Tamhane's tests at 5% significant level.
Results:
The surface roughness values of WO and WOG files significantly changed after use in root canals (p < 0.05). The used WOG files exhibited higher surface roughness change when compared with the used WO files (p < 0.05).
Conclusions:
Using WO and WOG Primary files in 3 root canals affected the surface topography of the files. After being used in root canals, the WOG files showed a higher level of surface porosity value than the WO files.
Related Concept Videos
Atomic Force Microscopy
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Atomic Orbitals
Intermolecular Forces
Hydrostatic Pressure Force on a Plane Surface
Hydrostatic Pressure Force on a Curved Surface
The Energies of Atomic Orbitals

