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First Description in Greece of mphC-Positive Staphylococci Causing Subclinical Mastitis in Ewes

Costas C Papagiannitsis1,2, Ergina Malli1, Katerina Tsilipounidaki1

  • 11 Department of Microbiology, University Hospital of Larissa , Larissa, Greece .

Microbial Drug Resistance (Larchmont, N.Y.)
|March 1, 2018
PubMed

Insights

The first mphC-positive staphylococci, Staphylococcus lentus and Staphylococcus xylosus, were identified in Greece. These resistant bacteria highlight the emergence of multidrug-resistant staphylococci in animals, posing economic challenges.

Area of Science:

  • Veterinary Microbiology
  • Antimicrobial Resistance
  • Genomics

Background:

  • Staphylococci are significant pathogens in animal infections.
  • Antimicrobial resistance in staphylococci is a growing concern globally.
  • The mphC gene confers resistance to macrolides, lincosamides, and streptogramin B antibiotics.

Purpose of the Study:

  • To report the first isolation of mphC-positive staphylococci from animal samples in Greece.
  • To characterize the genetic basis of antimicrobial resistance in these isolates.
  • To investigate the potential for horizontal gene transfer and independent acquisition of resistance genes.

Main Methods:

  • Isolation and identification of staphylococcal strains from animal origin.
  • Antimicrobial susceptibility testing using standard antibiotic panels.
  • Polymerase Chain Reaction (PCR) for detection of specific resistance genes (mphC, ermB).
  • Whole-genome sequencing (WGS) and de novo assembly for detailed genomic analysis.

Main Results:

  • Three mphC-positive staphylococcal isolates were identified: two Staphylococcus lentus and one Staphylococcus xylosus.
  • Isolates exhibited resistance to erythromycin and, in one case, lincomycin.
  • WGS revealed mphC in S. xylosus with similarity to known plasmids, and in S. lentus with similarity to strains from Tanzania, suggesting independent acquisition.
  • One isolate carried the ermB gene integrated via transposon Tn917.

Conclusions:

  • The emergence of mphC-positive staphylococci in animals in Greece is reported.
  • Independent acquisition events of the mphC gene contribute to the diversity of resistant staphylococci.
  • The presence of multiple resistance genes and elements like transposons can lead to multidrug-resistant staphylococci, impacting animal health and incurring economic losses.
Keywords:
Staphylococcus lentusStaphylococcus xylosusTn917ermBmacrolides

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