Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ammonia metabolism.

R L Tannen

    The American Journal of Physiology
    |October 1, 1978
    PubMed
    Summary

    The study examines how the body increases ammonia production during acidosis. The phosphate-dependent glutaminase pathway, particularly mitochondrial glutamine metabolism, is key, though other factors remain unclear.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Blueprint for discovery in academic medicine: plans, process and outcomes.

    Transactions of the American Clinical and Climatological Association·2001
    Same author

    The ethical issues of the Holmesburg studies have been addressed.

    Archives of dermatology·2000
    Same author

    Mechanisms of chronic hypoxia-induced renal cell growth.

    Kidney international·1999
    Same author

    Protection of acid-base balance by pH regulation of acid production.

    The New England journal of medicine·1998
    Same author

    Chronic hypoxia induces proliferation of cultured mesangial cells: role of calcium and protein kinase C.

    The American journal of physiology·1998
    Same author

    Chronic hypoxia induces LLC-PK1 cell proliferation and dedifferentiation by the activation of protein kinase C.

    The American journal of physiology·1997

    Area of Science:

    • Biochemistry
    • Renal Physiology
    • Metabolic Adaptations

    Background:

    • Acidosis triggers an adaptive increase in renal ammonia production.
    • Understanding these adaptive mechanisms is crucial for managing metabolic disorders.

    Purpose of the Study:

    • To elucidate the specific biochemical pathways and regulatory mechanisms responsible for increased ammonia production during acidosis.
    • To evaluate the relative importance of different glutaminase pathways and cellular compartments in this adaptive response.

    Main Methods:

    • Review and analysis of existing literature on ammonia metabolism in acidosis.
    • Examination of the roles of cytosolic and intramitochondrial pathways, including glutamine synthetase, glutaminase II, phosphate-independent glutaminase, and phosphate-dependent glutaminase.
    • Consideration of regulatory factors such as alpha-ketoglutarate and TCA cycle intermediates.

    Main Results:

    • Cytosolic pathways are unlikely to be primary drivers of adaptive ammonia production.
    • The intramitochondrial phosphate-dependent glutaminase pathway is considered of primary importance.
    • Adaptation may involve enhanced mitochondrial glutamine entry and/or phosphate-dependent glutaminase activation, with their relative contributions unresolved.
    • Decreased urine pH, rather than systemic pH, may be a critical factor.

    Conclusions:

    • The phosphate-dependent glutaminase pathway is central to the adaptive increase in ammonia production during acidosis.
    • Further research is needed to definitively resolve the roles of mitochondrial glutamine transport and enzyme activation.
    • The precise signaling mechanisms, including the role of urine pH and other potential factors, require further investigation.

    Related Experiment Videos