Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Enzymologic aspect of piperacillin combinations].

R Labia, A Morand, C Verchere-Beaur

    Presse Medicale (Paris, France : 1983)
    |December 20, 1986
    PubMed
    Summary

    Piperacillin shows susceptibility to beta-lactamases but is only slightly hydrolyzed by cephalosporinases. Combining piperacillin with amikacin effectively prevents the development of resistant Gram-negative bacilli mutants.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Ultrafast laser-written 3D nano-antenna for flux extraction and vertical focusing above near-infrared waveguides.

    Optics express·2026
    Same author

    Management of Tinea Capitis in children under 10 kg : Results from a French multicentre study.

    Journal of the European Academy of Dermatology and Venereology : JEADV·2025
    Same author

    Differences and similarities between Monkeypox and Chickenpox in children during an outbreak.

    Travel medicine and infectious disease·2024
    Same author

    Development of a 3D ultrafast laser written near-infrared spectro-interferometer.

    Optics letters·2023
    Same author

    Waveguide scattering antennas made by direct laser writing in bulk glass for spectrometry applications in the short-wave IR.

    Applied optics·2022
    Same author

    Real-life requests for Bordetella polymerase chain reaction testing in children presenting to hospital.

    Archives de pediatrie : organe officiel de la Societe francaise de pediatrie·2021

    Area of Science:

    • Microbiology
    • Pharmacology
    • Antibiotic Resistance

    Background:

    • Piperacillin, a penicillin antibiotic, exhibits susceptibility to various beta-lactamases, including those from Staphylococcus aureus and Gram-negative bacilli.
    • Its hydrolysis by cephalosporinases is minimal, similar to third-generation cephalosporins.
    • Inducible cephalosporinases produced by Enterobacter cloacae and Pseudomonas aeruginosa pose a challenge for antibiotic efficacy.

    Purpose of the Study:

    • To evaluate piperacillin's susceptibility to beta-lactamases and its inductive activity on cephalosporinases.
    • To assess the efficacy of combining piperacillin with amikacin or pefloxacin in preventing antibiotic resistance.

    Main Methods:

    • Testing piperacillin's susceptibility to penicillinase-type beta-lactamases.
    • Evaluating piperacillin's inductive activity on cephalosporinases from Enterobacter cloacae and Pseudomonas aeruginosa.
    • Assessing the effect of piperacillin-amikacin and piperacillin-pefloxacin combinations on the development of resistant mutants.

    Main Results:

    • Piperacillin demonstrated susceptibility to penicillinase-type beta-lactamases but minimal hydrolysis by cephalosporinases.
    • Piperacillin exhibited moderate inductive activity on cephalosporinases, with lower induction in Enterobacter compared to Pseudomonas.
    • The piperacillin-amikacin combination strongly inhibited beta-lactamase synthesis and prevented the emergence of resistant mutants in Enterobacter and Pseudomonas.
    • The piperacillin-pefloxacin combination showed efficacy against Enterobacter but was less effective against Pseudomonas due to easily acquired resistance.

    Conclusions:

    • Piperacillin's activity is influenced by beta-lactamase production, with varying inductive effects on cephalosporinases.
    • Combination therapy with amikacin is highly effective in preventing piperacillin resistance in Gram-negative bacilli.
    • Pefloxacin combination offers limited benefit against Pseudomonas resistance due to rapid in vitro development of resistance.

    Related Experiment Videos