Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Profile Leveling and Cross Sections01:26

Profile Leveling and Cross Sections

1.7K
Profile leveling and cross-sections are surveying methods used to determine and document terrain elevations for infrastructure projects such as highways, railroads, canals, and pipelines. These methods provide data for earthwork planning and alignment of proposed routes.  Profile leveling involves measuring elevations along a fixed line to create a vertical terrain profile. A surveyor sets up a leveling instrument at the benchmark (BM) and records a backsight (BS) to determine the...
1.7K
Method of Sections01:30

Method of Sections

1.3K
Consider a truss structure, as shown in the figure.
1.3K
Method of Sections: Problem Solving II01:30

Method of Sections: Problem Solving II

1.7K
Consider an arbitrary truss structure composed of diagonal, vertical, and horizontal members fixed to the wall. To calculate the force acting on members CB, GB, and GH, method of sections can be used. The loads and lengths of the horizontal and vertical members are known parameters, as shown in the figure.
1.7K
Method of Sections: Problem Solving I01:27

Method of Sections: Problem Solving I

1.1K
Consider a symmetrical roof truss structure, composed of vertical, diagonal, and horizontal members. The length of each horizontal member is 4 m. The lengths of the vertical members FB and HD are 4 m, while the length of member GC is 6 m. The loads acting at joints F, G, and H are 2 kN, while those at joints A and E are 1 kN.
1.1K
Sampling Methods: Sample Types01:18

Sampling Methods: Sample Types

3.3K
Sampling materials are classified into three main types: solid, liquid, and gas.
Solid samples include a variety of substances, such as sediments from water bodies, soil, metals, and biological tissues. Two standard methods for extracting sediments from water bodies are grab sampling and piston coring. Grab sampling involves using a device to collect a discrete sediment sample from the bottom of a water body with minimal disturbance. Grab samples do not always represent the entire area due to...
3.3K
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

7.3K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
7.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Humanin Mitigates Aβ-Induced Retinal Pigment Epithelium Injury via AMPK-Beclin1-Dependent Mitophagy.

Aging cell·2026
Same author

Pulmonary toxicity of micro- and nano polyethylene particles following intratracheal instillation in rats.

Particle and fibre toxicology·2026
Same author

Spatial multi-omics reveals region-specific molecular signatures in a 6-OHDA model of Parkinson's disease.

NPJ Parkinson's disease·2026
Same author

Developmental Exposure to Endocrine Disruptors and Persistent Pollutants Heightens Addiction Risk via Toxicological Mechanisms.

Biomolecules & therapeutics·2026
Same author

Interpretation of Photogenerated Charge Carrier Transfer Dynamics Using Interactive Surface-Molecular System.

ACS applied materials & interfaces·2026
Same author

High-throughput heart rate monitoring in Daphnia magna for sublethal ecotoxicological assessment.

Journal of hazardous materials·2026
Same journal

Fiber-reinforced hydrogels: From multiscale structural design to advanced engineering applications.

Biointerphases·2026
Same journal

Development and validation of a low-cost, direct-current-based biosensor for real-time monitoring of transendothelial electrical resistance in cell barriers.

Biointerphases·2026
Same journal

Biointerfaces in India.

Biointerphases·2026
Same journal

Biomimetic illumination enhancement inspired by guanine platelets in the photophore surface of the deep-sea bristlemouth Sigmops gracilis.

Biointerphases·2026
Same journal

Binding and orientation of ice nucleating proteins on hydrophilic and hydrophobic surfaces probed by photoelectron spectroscopies.

Biointerphases·2026
Same journal

Shell damage and mandible mechanics in the ant Messor wasmanni.

Biointerphases·2026
See all related articles

Related Experiment Video

Updated: Feb 13, 2026

A Hydrophobic Tissue Clearing Method for Rat Brain Tissue
08:24

A Hydrophobic Tissue Clearing Method for Rat Brain Tissue

Published on: December 23, 2020

4.6K

Molecular depth profiling on rat brain tissue sections prepared using different sampling methods.

Hyun Kyong Shon1, Shin Hye Kim2, Sohee Yoon1

  • 1Center for Nano-Bio Measurement, KRISS, Daejeon 34113, South Korea.

Biointerphases
|March 3, 2018
PubMed
Summary
This summary is machine-generated.

New methods for brain tissue preparation using time-of-flight secondary ion mass spectrometry (ToF-SIMS) prevent cholesterol migration, enabling accurate biomolecular imaging at room temperature. This technique aids neurodegenerative disease research.

More Related Videos

A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels
07:13

A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels

Published on: May 7, 2018

10.8K
Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples
09:38

Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples

Published on: November 23, 2016

20.0K

Related Experiment Videos

Last Updated: Feb 13, 2026

A Hydrophobic Tissue Clearing Method for Rat Brain Tissue
08:24

A Hydrophobic Tissue Clearing Method for Rat Brain Tissue

Published on: December 23, 2020

4.6K
A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels
07:13

A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels

Published on: May 7, 2018

10.8K
Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples
09:38

Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples

Published on: November 23, 2016

20.0K

Area of Science:

  • Biomedical Imaging
  • Neuroscience
  • Analytical Chemistry

Background:

  • Time-of-flight secondary ion mass spectrometry (ToF-SIMS) brain imaging is challenged by cholesterol migration, causing distorted biomolecular distributions, especially in white matter.
  • Current frozen-hydrated analysis requires continuous liquid nitrogen supply (-160°C), posing practical limitations.

Purpose of the Study:

  • To develop novel sample preparation and drying methods for accurate room-temperature biomolecular imaging of brain tissue using ToF-SIMS.
  • To overcome the cholesterol-induced matrix effect in brain ToF-SIMS analysis.

Main Methods:

  • A tape-supporting method was employed, placing rat brain tissue on a precooled stainless steel plate (-20°C).
  • The prepared tissue was freeze-dried within the ToF-SIMS load-lock chamber for approximately one hour before direct transfer to the main chamber.

Main Results:

  • Cholesterol migration to the surface was successfully inhibited in the corpus callosum (white matter) of the rat brain.
  • Sulfatide-related signals in the cerebellum were accurately detected without reduction in white matter, indicating successful preservation of biomolecular distribution.

Conclusions:

  • The proposed tape-supporting and freeze-drying method enables accurate, room-temperature biomolecular imaging of brain tissues using ToF-SIMS.
  • This technique offers a practical and effective tool for studying metabolites relevant to neurodegenerative diseases.