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Updated: Feb 13, 2026

Multispectral Optoacoustic Tomography for Functional Imaging in Vascular Research
Published on: June 8, 2022
Optimized lighting method of applying shaped-function signal for increasing the dynamic range of LED-multispectral
Xue Yang1, Yajia Hu1, Gang Li1
1Tianjin Key Laboratory of Biomedical Detecting Techniques and Instruments, Tianjin University, Tianjin 300072, China.
Abstract:
This paper proposes an optimized lighting method of applying a shaped-function signal for increasing the dynamic range of light emitting diode (LED)-multispectral imaging system. The optimized lighting method is based on the linear response zone of the analog-to-digital conversion (ADC) and the spectral response of the camera. The auxiliary light at a higher sensitivity-camera area is introduced to increase the A/D quantization levels that are within the linear response zone of ADC and improve the signal-to-noise ratio. The active light is modulated by the shaped-function signal to improve the gray-scale resolution of the image. And the auxiliary light is modulated by the constant intensity signal, which is easy to acquire the images under the active light irradiation. The least square method is employed to precisely extract the desired images. One wavelength in multispectral imaging based on LED illumination was taken as an example. It has been proven by experiments that the gray-scale resolution and the accuracy of information of the images acquired by the proposed method were both significantly improved. The optimum method opens up avenues for the hyperspectral imaging of biological tissue.
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