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Updated: Feb 13, 2026

Imaging Serotonergic Fibers in the Mouse Spinal Cord Using the CLARITY/CUBIC Technique
Published on: February 26, 2016
3D imaging in the postmortem human brain with CLARITY and CUBIC
Kunie Ando1, Quentin Laborde2, Jean-Pierre Brion3
1Laboratory of Histology, Neuroanatomy and Neuropathology, ULB Neuroscience Institute, Université Libre de Bruxelles, Brussels, Belgium; Laboratoire de Neuropathologie Escourolle, Hôpital de la Pitié-Salpêtrière, Paris, France.
Abstract:
Recent innovations in tissue clearing and imaging technologies have enabled us to analyse biological systems directly in three-dimensions using thick samples. In this review, we discuss two of these recently reported tissue-clearing technologies (CLARITY and CUBIC) that are compatible with archival formalin-fixed human brain materials that have been fixed in formalin for a long period of time. We will discuss the pros and cons of these two technologies, examples of visualisation of Alzheimer neuropathological hallmarks and the exact protocols that we regularly use in the laboratory.
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