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Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Updated: Jun 1, 2026

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

Published on: March 11, 2016

Fundus autofluorescence imaging.

Jodi Hwang¹, Hossein Ameri²

¹Department of Ophthalmology and Visual Sciences, John A. Moran Eye Center, University of Utah, Salt Lake City, UT, United States.

Handbook of Clinical Neurology

|May 30, 2026

Summary

This summary is machine-generated.

Fundus autofluorescence (FAF) imaging quickly assesses retinal health noninvasively. This technique maps retinal metabolism for diagnosing and managing various eye diseases.

Keywords:

Fundus autofluorescence Lipofuscin Near-infrared autofluorescence Retina Retinal pigment epithelium Short-wavelength autofluorescence

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Last Updated: Jun 1, 2026

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases

Published on: March 11, 2016

Autofluorescence Imaging to Evaluate Cellular Metabolism

Autofluorescence Imaging to Evaluate Cellular Metabolism

Published on: November 15, 2021

Autofluorescence Imaging to Evaluate Red Algae Physiology

Autofluorescence Imaging to Evaluate Red Algae Physiology

Published on: February 17, 2023

Area of Science:

Ophthalmology
Medical Imaging

Background:

Fundus autofluorescence (FAF) is a key imaging modality for retinal assessment.
It noninvasively evaluates photoreceptor and retinal pigment epithelium health.

Purpose of the Study:

To highlight the clinical utility of FAF in retinal disease management.
To discuss the role of FAF in topographic metabolic mapping of the retina.

Main Methods:

Utilizes short-wavelength autofluorescence, the most common FAF technique.
Considers advanced methods like near-infrared autofluorescence.

Main Results:

FAF enables precise topographic mapping of retinal metabolic profiles.
Facilitates early disease detection and prognosis assessment.

Conclusions:

FAF is crucial for diagnosing and managing retinal and choroidal diseases.
Advanced FAF techniques may enhance clinical applications.