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A Mouse Model for the Transition of Streptococcus pneumoniae from Colonizer to Pathogen upon Viral Co-Infection Recapitulates Age-Exacerbated Illness
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A Mouse Model for the Transition of Streptococcus pneumoniae from Colonizer to Pathogen upon Viral Co-Infection Recapitulates Age-Exacerbated Illness

Published on: September 28, 2022

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Whole genome analysis of a multidrug-resistant Streptococcus pneumoniae isolate from a patient with invasive

Yasuo Ohkoshi1, Toyotaka Sato2, Takayuki Wada3

  • 1Department of Microbiology, Sapporo Medical University School of Medicine, Sapporo, Japan; Department of Clinical Laboratory, NTT East Sapporo Hospital, Sapporo, Japan.

Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy
|March 3, 2018
PubMed

Insights

Multidrug-resistant Streptococcus pneumoniae strains exhibiting novel resistance mechanisms were identified in Japan. These strains rapidly evolved resistance through gene acquisition from other bacteria, posing a significant public health threat.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Emergence of multidrug-resistant (MDR) Streptococcus pneumoniae poses a global health challenge.
  • Streptococcus pneumoniae is a leading cause of bacterial pneumonia, meningitis, and sepsis.

Observation:

  • MDR Streptococcus pneumoniae strains with unique resistance profiles were isolated from a patient in Sapporo, Japan.
  • These isolates showed resistance to multiple antibiotics, including vancomycin, linezolid, daptomycin, carbapenems, and fluoroquinolones.
  • Genomic analysis revealed mutations in key resistance genes (parC, gyrA, pbp1a, pbp2a, pbp2b, pbp2x) and the presence of ermB and tetM resistance genes.

Findings:

  • The MDR Streptococcus pneumoniae strain (MDRSPN001) exhibited significant genetic divergence, with parC and pbp2x sequences resembling those of oral streptococci.
  • Evidence suggests rapid evolution of multidrug resistance through horizontal gene transfer and gene replacement, potentially involving Streptococcus mitis.
  • Identical serotypes (19F) and sequence types (ST10017) indicated a common origin for the isolated strains, with one previous isolate from 2014 showing macrolide susceptibility.

Implications:

  • The findings highlight the potential for rapid and drastic evolution of antibiotic resistance in Streptococcus pneumoniae through interspecies gene transfer.
  • Understanding these evolutionary mechanisms is crucial for developing effective strategies to combat MDR bacterial infections.
  • This study underscores the importance of continuous genomic surveillance for emerging resistance patterns in clinically significant pathogens.
Keywords:
Disseminated intravascular coagulationInvasive pneumococcal infectionMultidrug resistanceStreptococcus pneumoniaeWhole genome sequence

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