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Updated: Feb 13, 2026

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
Published on: February 16, 2017
Isolation and Characterization of Human Myoblast Culture In Vitro for Technologies of Cell and Gene Therapy of
V Yu Tabakov1, O E Zinov'eva2, O N Voskresenskaya2
1Research Centre for Medical Genetics, Moscow, Russia. vyutab@yandex.ru.
Abstract:
We analyzed cultures of 5 independent myoblast lines from human skeletal muscles. It was shown that the content of desmin-positive cells in cultures at early passages exceeds 90%. Typical morphofunctional signs of myogenic differentiation disturbances were identified and their dynamics was studied. Signs of alternative adipogenic and chondrogenic differentiation of cells were revealed. Based on these data, limitations for the use of myoblast cultures of certain passages for biomedical research and cell therapy were evaluated.
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