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Undecalcified Bone Preparation for Histology, Histomorphometry and Fluorochrome Analysis
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Screening Gene Knockout Mice for Variation in Bone Mass: Analysis by μCT and Histomorphometry
David W Rowe1, Douglas J Adams2, Seung-Hyun Hong3
1Regenerative Medicine and Skeletal Development, Department of Reconstructive Sciences, Biomaterials and Skeletal Development, School of Dental Medicine, University of Connecticut Health, Farmington, CT, 06030, USA. drowe@uchc.edu.
Current Osteoporosis Reports
|March 7, 2018
Summary
The International Mouse Phenotyping Consortium (IMPC) uses micro-computed tomography (μCT) to identify novel skeletal variations in gene knockout mice. This approach reveals genetic influences on bone health and disease.
Area of Science:
- Skeletal Biology
- Genetics
- Biomedical Research
Background:
- The International Mouse Phenotyping Consortium (IMPC) generates gene knockout mouse lines for comprehensive phenotyping.
- Understanding the genetic basis of skeletal health and disease requires detailed analysis of bone morphology and cellularity.
Purpose of the Study:
- To present a phenotyping program utilizing micro-computed tomography (μCT) for assessing distal femur and vertebra in mouse models.
- To identify novel skeletal variations not detected by standard IMPC screening.
- To integrate μCT and bone histomorphometric data for a comprehensive understanding of skeletal phenotypes.
Main Methods:
- Micro-computed tomography (μCT) scans of distal femur and vertebra were performed on gene knockout mouse lines.
- Lines exhibiting significant μCT variations underwent computer-based bone histomorphometric analysis.
- Data was organized and presented on the www.bonebase.org web portal for accessibility.
Main Results:
- Approximately 15% of the 220 examined lines showed significant μCT variations, many missed by initial IMPC screens.
- Sexual dimorphism and variations between bone compartments added complexity to skeletal findings.
- The web portal effectively groups lines with similar morphological features and presents integrated histological data.
Conclusions:
- μCT-based phenotyping is effective in identifying novel skeletal variations in knockout mouse lines.
- Integrated morphological and histological data provide a valuable resource for skeletal biologists and clinicians.
- This approach aids in understanding the polygenic basis of degenerative bone diseases.
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