Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imprinting01:22

Imprinting

11.2K
Behavioral imprinting is observed in some newborn animals and occurs when they develop strong and specific attachments to another animal (usually a parent) following brief, early-life exposures. Offspring imprint onto parents within a brief period after birth or hatching; this time window is called the critical period. Once imprinting occurs, the bond established between the parents and their offspring is usually long-lasting.
11.2K
RNA-seq03:21

RNA-seq

12.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.1K
Epigenetic Regulation01:46

Epigenetic Regulation

33.9K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.9K
Epigenetic Regulation01:37

Epigenetic Regulation

3.9K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.9K
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

37.3K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
37.3K
Analysis of Population Pharmacokinetic Data01:12

Analysis of Population Pharmacokinetic Data

815
Analysis of population pharmacokinetic data involves studying the behavior of drugs within diverse populations to understand their pharmacokinetic parameters. Traditional pharmacokinetic methods typically involve collecting samples from a few individuals and estimating these parameters. While these methods are commonly used, they have limitations in capturing the variability in drug response among individuals or heterogeneous populations. Population pharmacokinetics is employed to address these...
815

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HPV Serology and Circulating Viral DNA for Detection, Genotyping, and Measurement of Disease Burden in Oropharyngeal Cancer.

International journal of cancer·2026
Same author

Circulating Cell-Free DNA Methylation Profiling Enables Detection, Distinction, and Estrogen Receptor Status Classification of Advanced Breast Cancer.

Cancer research·2026
Same author

Erratum to: Distinct immune escape and microenvironment between RG-like and pri-OPC-like glioma revealed by single-cell RNA-seq analysis.

MedScience·2026
Same author

Haploid Mutation Mapping Identifies a Homoeologous Non-Reciprocal Translocation Linked to Reduced Fibre and Enhanced Protein in Brassica napus.

Plant biotechnology journal·2026
Same author

A new method for determining the pore water pressure around twin shallow circular tunnels.

Scientific reports·2025
Same author

A stomata imaging and segmentation pipeline incorporating generative AI to reduce dependency on manual groundtruthing.

Plant methods·2025
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Feb 13, 2026

ATAC-Seq Optimization for Cancer Epigenetics Research
07:13

ATAC-Seq Optimization for Cancer Epigenetics Research

Published on: June 30, 2022

5.4K

A Protocol for Epigenetic Imprinting Analysis with RNA-Seq Data.

Jinfeng Zou1, Daoquan Xiang2, Raju Datla2

  • 1National Research Council Canada, Montreal, QC, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|March 7, 2018
PubMed
Summary
This summary is machine-generated.

Researchers developed a new RNA-Seq protocol to study genomic imprinting in plants. This method analyzes parent-of-origin gene expression during early embryo development in Arabidopsis thaliana.

Keywords:
Arabidopsis thalianaGenomic imprintingMaternal and paternal contributionsRNA-Seq

More Related Videos

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

6.5K
Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
06:22

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

Published on: August 25, 2020

13.5K

Related Experiment Videos

Last Updated: Feb 13, 2026

ATAC-Seq Optimization for Cancer Epigenetics Research
07:13

ATAC-Seq Optimization for Cancer Epigenetics Research

Published on: June 30, 2022

5.4K
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

6.5K
Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
06:22

Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq

Published on: August 25, 2020

13.5K

Area of Science:

  • Epigenetics and Plant Development
  • Genomic Imprinting Mechanisms
  • Transcriptome Analysis

Background:

  • Genomic imprinting is an epigenetic process controlling parent-specific gene expression.
  • Imprinted genes are crucial for embryonic and seed development in plants, with roles in disease.
  • The molecular basis of imprinting in plants requires further investigation.

Purpose of the Study:

  • To investigate gene expression patterns related to parent of origin in Arabidopsis thaliana.
  • To explore the implications of parent-specific gene expression in embryo and seed development.
  • To develop and validate a protocol for analyzing epigenetic imprinting using RNA-Seq.

Main Methods:

  • Generated RNA-Seq transcriptome data from early Arabidopsis thaliana embryos (F1s) from a Col-0 x Tsu-1 cross.
  • Developed a protocol to evaluate maternal and paternal gene contributions.
  • The protocol accounts for tissue contamination, sequencing quality, read processing, variant calling, and parent-of-origin-specific SNPs.

Main Results:

  • Successfully generated RNA-Seq datasets for early embryo development.
  • Established a robust protocol for assessing parental gene contributions in plants.
  • The protocol enables accurate inference of parental contributions based on expressed genes and SNPs.

Conclusions:

  • The developed RNA-Seq based protocol provides a systematic approach to study epigenetic imprinting in plants.
  • This methodology can be applied to model and crop plants for evaluating imprinting effects.
  • Advances in RNA-Seq enable whole-genome analysis of epigenetic imprinting.