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CSF N-Glycomics Using MALDI MS Techniques in Alzheimer's Disease
Angelo Palmigiano1, Angela Messina1, Rosaria Ornella Bua1
1CNR, Istituto per i Polimeri, Compositi e i Biomateriali Catania, Catania, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|March 8, 2018
Summary
This study details a method for analyzing cerebrospinal fluid (CSF) N-glycans using mass spectrometry. The optimized technique successfully linked specific N-glycan profiles to Alzheimer
Area of Science:
- Biochemistry
- Analytical Chemistry
- Neuroscience
Background:
- Cerebrospinal fluid (CSF) N-glycans offer potential biomarkers for neurological diseases.
- Structural elucidation of the N-glycome is crucial for understanding disease mechanisms.
Purpose of the Study:
- To present a robust methodology for the structural elucidation of the CSF N-glycome.
- To optimize N-glycan analysis for high-quality mass spectrometry data.
Main Methods:
- N-glycans released from glycoproteins using peptide-N-glycosidase F (PNGase F).
- Purification via C18 and porous graphitized carbon (PGC) solid-phase extraction (SPE).
- Permethylation followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis.
Main Results:
- Optimized protocol yields high-quality mass spectra with excellent signal-to-noise ratio up to m/z 4800.
- Method successfully applied to differentiate N-glycome profiles in early and advanced Alzheimer's disease.
Conclusions:
- The presented methodology provides a reliable approach for CSF N-glycome structural elucidation.
- This technique can identify specific N-glycan signatures associated with Alzheimer's disease progression.