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In Vivo Two-Photon Calcium Imaging of Hippocampal Neurons in Alzheimer Mouse Models

Marc Aurel Busche1,2,3

  • 1Department of Neurology, Massachusetts General Hospital, Harvard Medical School, MassGeneral Institute for Neurodegenerative Disease, Charlestown, MA, USA. MABusche@mgh.harvard.edu.

Methods in Molecular Biology (Clifton, N.J.)
|March 8, 2018
PubMed

Insights

This study presents a new method for in vivo two-photon calcium imaging in Alzheimer

Area of Science:

  • Neuroscience
  • Biomedical Imaging
  • Alzheimer's Disease Research

Background:

  • In vivo two-photon microscopy has advanced Alzheimer's disease (AD) research by enabling visualization of amyloid plaques and therapy effects in live mouse models.
  • Previous studies using this technique revealed amyloid-dependent neuronal hyperactivity, a finding not evident from in vitro analyses.

Purpose of the Study:

  • To present a novel method for high-resolution in vivo two-photon calcium imaging in the hippocampus of Alzheimer's disease mouse models.
  • To achieve single-cell and single-action potential accuracy for studying neuronal activity in AD.

Main Methods:

  • Utilizing in vivo two-photon microscopy combined with fluorescent calcium indicators.
  • Implementing advanced imaging techniques for high-accuracy calcium signal detection in hippocampal neurons of AD mouse models.

Main Results:

  • The presented method allows for precise in vivo imaging of neuronal activity in the hippocampus.
  • This technique offers single-cell and single-action potential resolution, crucial for understanding AD-related neuronal dysfunction.

Conclusions:

  • The developed method enhances the capability to study Alzheimer's disease mechanisms at a cellular level in vivo.
  • This advancement facilitates a deeper understanding of neuronal impairments and potential therapeutic targets in Alzheimer's disease.
Keywords:
Alzheimer’s diseaseAmyloid-βCalcium imagingHippocampusIn vivo imagingMouse modelsNeuronal hyperactivityTwo-photon microscopy

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