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Updated: Feb 13, 2026

Author Spotlight: Generating Neuronal Phenotypic Profiles - A Protocol to Culture and Image Human Midbrain Dopaminergic Neurons
Published on: July 7, 2023
hPSC-derived Midbrain Dopaminergic Neurons Generated in a Scalable 3-D Biomaterial
Maroof M Adil1, David V Schaffer1
1Departments of Chemical and Biomolecular Engineering, Molecular and Cell Biology, Bioengineering, and Helen Wills Neuroscience Institute, University of California, Berkeley, California.
Abstract:
Human pluripotent stem cell (hPSC)-derived midbrain dopaminergic (mDA) neurons may facilitate the development of therapies for Parkinson's disease via disease modeling, drug screening, and cell replacement therapy. However, large numbers of cells are typically needed for these applications, and 2-D culture-based approaches typically used for mDA differentiation are difficult to scale up and require a long time for mDA maturation. Here we present a protocol to rapidly generate functional mDA neurons in a fully defined, scalable, thermoresponsive 3-D biomaterial. Resource-efficient and accelerated differentiation of large numbers of mDA neurons may thus facilitate studying and treating PD. © 2018 by John Wiley & Sons, Inc.
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