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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
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Functional groups are a group of atoms with characteristic properties, which when linked to the carbon skeleton of a molecule, alter the properties of that molecule. For example, certain functional groups will make a molecule hydrophilic, whereas others will make them hydrophobic. These functional groups are an indispensable part of organic chemistry and important components of biological molecules, such as carbohydrates, proteins, lipids, and nucleic acids. Each functional group is a unique...
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Pharmacokinetics is a scientific discipline that focuses on the journey of a drug within the body, encompassing four key stages: absorption, distribution, metabolism, and elimination. The first stage, absorption, involves the drug's transfer into the bloodstream. Several factors dictate the extent and speed of this process. For example, the liver often metabolizes oral drugs before they reach systemic circulation, leading to only partial absorption. In contrast, intravenous (IV)...
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Bioavailability refers to the proportion of an unaltered drug that, after administration, enters the systemic circulation and can be distributed to the desired action site. Factors such as gastrointestinal (GI) absorption and liver biotransformation influence the bioavailability of a drug when it is administered orally. When a drug is administered intravenously, it enters the systemic circulation directly; by definition, its bioavailability is assumed to be 100%. The bioavailability of an...
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Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence
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Flow Cytometry: An Overview.

Katherine M McKinnon1

  • 1Vaccine Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland.

Current Protocols in Immunology
|March 8, 2018
PubMed
Summary
This summary is machine-generated.

Flow cytometry analyzes single cells using lasers and fluorescent reagents. This powerful technology enables detailed studies in immunology, cancer biology, and infectious diseases.

Keywords:
flow cytometryfluorescencelight scatterreagents

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Area of Science:

  • Cell biology
  • Biotechnology

Background:

  • Flow cytometry is a key technology for rapid, multi-parametric single-cell analysis.
  • It employs lasers to detect scattered and fluorescent light signals from cells.

Purpose of the Study:

  • To describe the principles and applications of flow cytometry.
  • To highlight its advancements and utility in various biological fields.

Main Methods:

  • Utilizes lasers for light sources.
  • Detects scattered and fluorescent light signals using photodiodes or photomultiplier tubes.
  • Converts light signals to electronic data for computer analysis and .fcs file storage.

Main Results:

  • Enables analysis and purification of cell populations based on light scattering and fluorescence.
  • Employs diverse fluorescent reagents like antibodies, nucleic acid dyes, and viability dyes.
  • Facilitates detailed studies of the immune system and other cell biology areas.

Conclusions:

  • Flow cytometry is a versatile and powerful tool with broad applications.
  • Recent advancements have significantly enhanced its capability for detailed biological research.