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Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA
Published on: June 25, 2014
Chromium resistance genetic element flanked by XerC/XerD recombination sites and its distribution in environmental
Sofia Mindlin1, Anatoliy Petrenko1, Mayya Petrova1
1Russian Academy of Sciences, Institute of Molecular Genetics, Kurchatov sq. 2, Moscow 123182, Russia.
Abstract:
A novel mobile genetic element has been identified in small plasmids isolated from permafrost strains of Acinetobacter lwoffii. This element, designated the chrAB dif module, contains the chromium resistance genes chrA and chrB, functionally active both in the original host strains and after transfer into Acinetobacter baylyi. The 3011 bp chrAB dif module is flanked by XerC/XerD recombination sites highly homologous to those of the site-specific recombination system dif/Xer. Analysis of public databases revealed almost identical variants of the chrAB dif module in different plasmids in strains of various Acinetobacter species predominantly inhabiting the environment (A. lwoffii, Acinetobacter indicus, Acinetobacter idrijaensis, Acinetobacter shindleri and Acinetobacter nosocomialis). Together with previously described Acinetobacter antibiotic resistance elements, the chrAB dif module defines a new group of mobile elements that rely on the dif/Xer system for their mobility. Our observations suggest an ancient origin of the mobile elements flanked by dif sites and their participation in the mobilization of plasmid genes bearing adaptive functions.
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