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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

Cytoplasmic functions of long noncoding RNAs.

Ji Heon Noh¹, Kyoung Mi Kim¹, Waverly G McClusky¹

¹Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland.

Wiley Interdisciplinary Reviews. RNA

|March 9, 2018

Summary

This summary is machine-generated.

Long noncoding RNAs (lncRNAs) in the cytoplasm regulate gene expression through interactions with proteins and nucleic acids. This review explores their roles in mRNA turnover, translation, and signaling pathways.

Keywords:

circRNA lncRNA miRNA noncoding RNA post-transcriptional gene regulation ribonucleoprotein complexes

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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

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Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

Published on: April 7, 2023

Area of Science:

Molecular Biology
Genetics
Cell Biology

Background:

Long noncoding RNAs (lncRNAs) are key regulators of gene expression.
Nuclear lncRNAs are well-characterized for their roles in gene organization and transcription.
Cytoplasmic lncRNAs are less understood but play crucial roles in cellular processes.

Purpose of the Study:

To review the current knowledge of cytoplasmic lncRNAs.
To elucidate the diverse regulatory functions of cytoplasmic lncRNA complexes.
To highlight future research directions and therapeutic potential.

Main Methods:

Literature review of studies on cytoplasmic lncRNAs.
Analysis of lncRNA interactions with RNA-binding proteins and nucleic acids.
Synthesis of findings on lncRNA-mediated gene regulation.

Main Results:

Cytoplasmic lncRNAs form complexes with proteins and nucleic acids.
These complexes regulate mRNA turnover, translation, and protein stability.
Cytoplasmic lncRNAs modulate signaling pathways and sponge cytosolic factors.

Conclusions:

Cytoplasmic lncRNAs are critical regulators of gene expression at multiple post-transcriptional levels.
Further research is needed to fully understand their biological impact.
lncRNA-centered therapeutic strategies hold significant promise.