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Updated: Feb 13, 2026

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
Published on: April 24, 2020
Interrelationships between changes in erythropoietin, plasma volume, haemoglobin concentration, and total haemoglobin
Dariusz Sitkowski1, Zbigniew Szygula2, Andrzej Pokrywka3
1a Department of Physiology , Institute of Sport - National Research Institute , Warsaw , Poland.
Abstract:
Interrelationships between physiological changes (Δ) in erythropoietin (EPO), plasma volume (PV), haemoglobin concentration ([Hb]), and total haemoglobin mass (tHb-mass) were examined in cyclists who trained in different altitudes. Regardless of differences in pattern of changes observed in three training locations, ΔEPO was correlated positively with ΔPV, negatively with Δ[Hb], and trivially with ΔtHb-mass. Δ[Hb] was negatively correlated with ΔPV. In the pooled data the Spearman's rank correlation coefficients were as follows: r = 0.783, P < 0.001; r = -0.704, P < 0.001; r = 0.136, P > 0.05; r = -0.813, P < 0.001, respectively. The obtained results have shown that EPO does not only regulate [Hb] by erythropoiesis stimulation but also by PV modulation, which probably aims at keeping proper level of arterial oxygen content for oxygen delivery to tissues.
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