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Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
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Catch the live show: Visualizing damaged DNA in vivo

Roxanne Oshidari1, Karim Mekhail2

  • 1Department of Laboratory Medicine and Pathobiology, University of Toronto, MaRS Centre, West Tower, 661 University Avenue, Toronto, Ontario M5G 1M1, Canada.

Methods (San Diego, Calif.)
|March 9, 2018
PubMed

Insights

Understanding DNA repair in living cells is crucial for organism health. This review covers methods to visualize DNA damage and repair dynamics in real-time using advanced live-cell imaging and genome editing techniques.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Organismal health is dependent on effective DNA repair mechanisms.
  • DNA repair processes are dynamic and require real-time observation.
  • Characterizing DNA repair in live cells is essential for understanding cellular responses to damage.

Purpose of the Study:

  • To review established and recent methods for inducing DNA damage in live cells.
  • To summarize techniques for visualizing DNA damage and repair in real-time.
  • To highlight the importance of live-cell imaging for studying DNA repair dynamics.

Main Methods:

  • Utilizing advanced genome editing tools.
  • Employing live-cell imaging approaches.
  • Summarizing methods for inducing and visualizing DNA damage and repair.

Main Results:

  • Established methods for DNA damage induction and visualization are presented.
  • Recent advancements in live-cell imaging for DNA repair studies are discussed.
  • The dynamic nature of DNA repair factors in response to damage is highlighted.

Conclusions:

  • Live-cell characterization of DNA repair is vital for understanding cellular health.
  • Advanced imaging and genome editing enable real-time visualization of DNA repair.
  • This review provides a comprehensive overview of current methodologies in the field.

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