Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What are Viruses?00:50

What are Viruses?

128.4K
Overview
128.4K
Multiple Allele Traits01:49

Multiple Allele Traits

38.2K
The Concept of Multiple Allelism
38.2K
Factors Affecting Protein-Drug Binding: Drug Interactions01:23

Factors Affecting Protein-Drug Binding: Drug Interactions

617
Drug interactions are a critical aspect of pharmacology and can occur when two or more drugs compete for the same binding site. This competition can result in one drug displacing another, altering the effect of the displaced drug. Drug interactions are complex processes that rely heavily on how much of the displacer drug is present and how strongly it can bind to the same sites as the displaced drug.
Displacement interactions can have varying outcomes, ranging from toxicity to virtually...
617
Protein Complex Assembly02:41

Protein Complex Assembly

16.9K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.9K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

26.5K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
26.5K
Protein Networks02:26

Protein Networks

4.6K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neutrophil extracellular traps induced by activated platelets as a cause of neutrophil-platelet aggregation in β-thalassaemia/haemoglobin E patients.

British journal of haematology·2026
Same author

Plant-Produced Broadly Neutralizing Influenza Monoclonal Antibody CR9114 Exhibits Activity Against Heterologous Avian Influenza Viruses.

Vaccines·2026
Same author

Evaluation of a Cell-Adapted Live Attenuated African Swine Fever Virus Thai-Strain Vaccine Candidate: Highlighting Enhanced Virulence Risk in Co-Infected Pigs.

Vaccines·2025
Same author

Increased expression of chaperone proteins in response to DENV 2 infection of Huh-7 liver cells.

PloS one·2025
Same author

Comparative Analysis of Two Zika Virus Isolates in a Rhesus Macaque Pregnancy Model.

Viruses·2025
Same author

Effects of the fatty acid synthase inhibitors triclosan and lapatinib on dengue virus and Zika virus infection.

Scientific reports·2025

Related Experiment Video

Updated: Feb 13, 2026

Visualizing Dengue Virus through Alexa Fluor Labeling
09:11

Visualizing Dengue Virus through Alexa Fluor Labeling

Published on: July 9, 2011

14.4K

Hsp90 interacts with multiple dengue virus 2 proteins.

Kanjana Srisutthisamphan1, Krit Jirakanwisal1, Suwipa Ramphan1

  • 1Institute of Molecular Biosciences, Mahidol University, Bangkok, Thailand.

Scientific Reports
|March 11, 2018
PubMed
Summary

Heat shock protein 90 (Hsp90) interacts with dengue virus (DENV) proteins, but inhibiting Hsp90 shows a slight anti-viral effect, not a proviral role, in DENV replication.

More Related Videos

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
09:39

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Published on: March 31, 2022

3.7K
A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
04:23

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

Published on: April 28, 2019

7.2K

Related Experiment Videos

Last Updated: Feb 13, 2026

Visualizing Dengue Virus through Alexa Fluor Labeling
09:11

Visualizing Dengue Virus through Alexa Fluor Labeling

Published on: July 9, 2011

14.4K
Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
09:39

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Published on: March 31, 2022

3.7K
A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
04:23

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

Published on: April 28, 2019

7.2K

Area of Science:

  • Virology
  • Cellular Biology
  • Molecular Biology

Background:

  • Dengue virus (DENV) infections pose a significant global health threat.
  • No effective antiviral therapies currently exist for DENV.
  • Heat shock protein 90 (Hsp90), a cellular chaperone, often aids viral replication.

Purpose of the Study:

  • To investigate the potential role of Hsp90 in DENV replication.
  • To determine interactions between Hsp90 and DENV viral proteins.

Main Methods:

  • Co-immunoprecipitation assays were used to identify DENV protein interactions with Hsp90.
  • Colocalization studies examined the spatial relationship between Hsp90 and DENV proteins within cells.
  • Hsp90 activity was inhibited to assess its impact on DENV replication.

Main Results:

  • Six DENV proteins (E, NS1, NS2B, NS3, NS4B, NS5) were found to interact with Hsp90.
  • Four DENV proteins (E, NS1, NS3, NS5) colocalized with Hsp90.
  • Hsp90 inhibition led to decreased cellular E protein, increased extracellular E protein, and higher virus titers, suggesting a minor anti-DENV effect.

Conclusions:

  • Hsp90 interacts with multiple DENV proteins.
  • Contrary to expectations for other viruses, Hsp90 exhibits a slight anti-viral effect in DENV infection.
  • Further research into Hsp90's role could inform DENV therapeutic strategies.